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. 2021 Mar;21(3):181.
doi: 10.3892/ol.2021.12442. Epub 2021 Jan 6.

Sarcoma-180 tumor affects the quality of oocytes in mice

Zihang Chen  1 , Simin Wang  1 , Xuexia Luo  1 , Yanhong Yang  1
Affiliations

Sarcoma-180 tumor affects the quality of oocytes in mice

Zihang Chen et al. Oncol Lett. 2021 Mar.

Abstract

Numerous factors can affect the quality of oocytes; however, the effects of cancer on the quality of oocytes and the underlying mechanisms remain unclear. In the present study, the effects of the sarcoma-180 (S-180) cell line on the quality of oocytes were investigated using S-180 tumor-bearing mice. In total, 42 female C57BL/6J mice were randomly divided into the tumor-bearing group and the control group, with 21 mice per group. The weight of the mice and ovaries were recorded, and blood glucose, serum insulin, lipopolysaccharide, triglyceride (TG) and total cholesterol (TC) levels were analyzed using the corresponding detection kits. Hematoxylin and eosin staining was performed to observe the pathological changes of the ovarian tissue, and reverse transcription-quantitative PCR (RT-qPCR) was used to analyze the expression levels of meiosis arrest female 1 (MARF1), SUMO-specific protease 7 (SENP7), aralkylamine N-acetyltransferase (AANAT), cell division cycle 25B and glycine-rich protein 3. The results of the present study revealed that the number of oocytes in the two groups of mice was similar; however, the number of abnormal oocytes was increased in the tumor-bearing group. The serum levels of TG and TC were significantly elevated in the tumor-bearing group compared with in the control group (P<0.01). Additionally, RT-qPCR analysis demonstrated that the expression levels of SENP7 were downregulated, while the expression levels of MARF1 and AANAT were upregulated in the ovaries of the tumor-bearing group compared with in the control group (P<0.01). In conclusion, the findings of the present study suggested that cancer may affect the reproductive system of mice and decrease the quality of oocytes by regulating the expression levels of reproduction-associated genes. These results provided novel insights into the reproductive ability of patients with cancer.

Keywords: Sarcoma-180; oocyte; quality; tumor-bearing mice.

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Figures

Figure 1.
Figure 1.
Ovary weight and serum analysis. (A) Ovary weight. Levels of (B) blood glucose, (C) serum insulin, (D) serum LPS, (E) serum TG and (F) serum TC. **P<0.01. LPS, lipopolysaccharide; TG, triglyceride; TC, total cholesterol.
Figure 2.
Figure 2.
Hematoxylin and eosin staining of ovarian tissues. Ovary of the control group at (A) ×100 and (B) ×200 magnification. Ovary of the tumor-bearing group at (C) ×100 and (D) ×200 magnification. The red arrow indicates a mature follicle; the pink arrow indicates an abnormal follicle.
Figure 3.
Figure 3.
Observation of the morphology of oocytes. Oocytes of the control group at (A) ×100, (B) ×200 and (C) ×400 magnification. Oocytes of the tumor-bearing group at (D) ×100, (E) ×200 and (F) ×400 magnification. The red arrow indicates a normal oocyte; the pink arrow indicates an abnormal oocyte.
Figure 4.
Figure 4.
Expression levels of reproduction-associated genes in ovaries analyzed by reverse transcription-quantitative PCR. Expression levels of (A) SENP7, (B) MARF1, (C) AANAT, (D) CDC25B and (E) GRP3. *P<0.05. SENP7, SUMO-specific protease 7; MARF1, meiosis arrest female 1; AANAT, aralkylamine N-acetyltransferase; CDC25B, cell division cycle 25B; GRP3, glycine-rich protein 3.
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