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. 2021 Jun;43(6):1175-1182.
doi: 10.1007/s10529-021-03099-w. Epub 2021 Feb 11.

Continuous production of fructooligosaccharides by recycling of the thermal-stable β-fructofuranosidase produced by Aspergillus niger

Juan Wang #  1 , Jing Zhang #  1 , Lu Wang  1 , Hong Liu  1 , Ning Li  2 , Huanxia Zhou  2 , Zhanguo Ning  2 , Weican Zhang  1 , Lushan Wang  1 , Feng Huang  3 , Yaohua Zhong  4
Affiliations

Continuous production of fructooligosaccharides by recycling of the thermal-stable β-fructofuranosidase produced by Aspergillus niger

Juan Wang et al. Biotechnol Lett. 2021 Jun.

Abstract

Objective: To achieve continuous production of fructooligosaccharides (FOS) by recycling of the mycelial cells containing the thermal-stable β-fructofuranosidase in Aspergillus niger without immobilization.

Results: The thermal-stable β-fructofuranosidase FopA-V1 was successfully expressed in A. niger ATCC 20611 under the control of the constitutive promoter PgpdA. The engineered A. niger strain FV1-11 produced the β-fructofuranosidase with improved thermostability, which remained 91.2% of initial activity at 50 °C for 30 h. Then its mycelial β-fructofuranosidase was recycled for the synthesis of FOS. It was found that the enzyme still had 79.3% of initial activity after being reused for six consecutive cycles, whereas only 62.3% β-fructofuranosidase activity was detected in the parental strain ATCC 20611. Meanwhile, the FOS yield of FV1-11 after six consecutive cycles reached 57.1% (w/w), but only 51.0% FOS yield was detected in ATCC 20611.

Conclusions: The thermal-stable β-fructofuranosidase produced by A. niger can be recycled to achieve continuous synthesis of FOS with high efficiency, providing a powerful and economical strategy for the industrial production of FOS.

Keywords: Aspergillus niger; Continuous production; Fructooligosaccharides (FOS); Thermostability; β-fructofuranosidase.

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