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. 2021 May;34(3):862-877.
doi: 10.1007/s13577-021-00486-3. Epub 2021 Feb 12.

Transcriptomic analysis reveals tumor stage- or grade-dependent expression of miRNAs in serous ovarian cancer

Caglar Berkel  1 , Ercan Cacan  2
Affiliations

Transcriptomic analysis reveals tumor stage- or grade-dependent expression of miRNAs in serous ovarian cancer

Caglar Berkel et al. Hum Cell. 2021 May.

Abstract

Ovarian cancer (OC) is the most lethal gynecological malignancy and cellular mechanisms regulating OC progression are not completely understood. miRNAs are involved in many signaling pathways which are critical for the progression of malignant tumors, including OC. In the present study, we aim to identify miRNAs whose expression change in a tumor stage- and/or grade-dependent manner in serous OC. Computational analysis was performed in R using The Cancer Genome Atlas miRNA dataset. Kaplan-Meier plots were constructed to compare the survival of patients with low and high expressions of identified miRNAs. We found that 91 and 90 miRNAs out of 799 are differentially expressed in terms of tumor stage and grade, respectively. miR-152, miR-375 and miR-204 were top three hits in terms of tumor stage; and similarly, miR-125b, miR-768-5p and -3p in terms of tumor grade. Among top 15 miRNAs whose expression most significantly changed between tumor stages, 66.7% were upregulated in late stage. However, 53.3% of top 15 miRNAs identified in terms of tumor grade were upregulated in high grade. 11 miRNAs are differentially expressed in terms of both tumor stage and grade. Expression changes of some of the top miRNAs were found to be associated with shorter survival in serous OC. Text mining analysis showed that most of these miRNAs have not been previously studied in the context of OC. Mechanistic studies of these miRNAs in OC progression, differentiation and metastasis will be of high importance to develop novel strategies for the treatment of serous ovarian cancer.

Keywords: Cancer progression; Serous ovarian cancer; Transcriptomics; Tumor grade; Tumor stage; miRNA.

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References

    1. Deb B, Uddin A, Chakraborty S. miRNAs and ovarian cancer: an overview. J Cell Physiol. 2018;233(5):3846–54. https://doi.org/10.1002/jcp.26095 (Epub 2017 Aug 25 PMID: 28703277). - DOI - PubMed
    1. Berkel C, Kucuk B, Usta M, Yilmaz E, Cacan E. The effect of Olaparib and Bortezomib combination treatment on ovarian cancer cell lines. Eur J Biol. 2020. https://doi.org/10.26650/EurJBiol.2020.0035 . - DOI
    1. Berkel C, Cacan E. In silico analysis of DYNLL1 expression in ovarian cancer chemoresistance. Cell Biol Int. 2020;44(8):1598–605. https://doi.org/10.1002/cbin.11352 (Epub 2020 Apr 13 PMID: 32208526). - DOI - PubMed
    1. Della Pepa C, Tonini G, Pisano C, Di Napoli M, Cecere SC, Tambaro R, Facchini G, Pignata S. Ovarian cancer standard of care: are there real alternatives? Chin J Cancer. 2015;34(1):17–27. https://doi.org/10.5732/cjc.014.10274 (PMID: 25556615; PMCID: PMC4302086). - DOI - PubMed
    1. Aboutalebi H, Bahrami A, Soleimani A, Saeedi N, Rahmani F, Khazaei M, Fiuji H, Shafiee M, Ferns GA, Avan A, Hassanian SM. The diagnostic, prognostic and therapeutic potential of circulating microRNAs in ovarian cancer. Int J Biochem Cell Biol. 2020;124:105765. https://doi.org/10.1016/j.biocel.2020.105765 (Epub 2020 May 17 PMID: 32428568). - DOI - PubMed

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