Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021:2235:139-153.
doi: 10.1007/978-1-0716-1056-5_10.

Manipulating Pericyte Function with MicroRNAs

Milena Vitiello  1 , Ben Cathcart  1 , Andrea Caporali  1 , Marco Meloni  2   3
Affiliations

Manipulating Pericyte Function with MicroRNAs

Milena Vitiello et al. Methods Mol Biol. 2021.

Abstract

MicroRNAs (miRNAs) are expressed in all cell types, including pericytes, and play essential roles in vascular development, homeostasis, and disease. Manipulation of pericytes with miRNA mimics and inhibitors represents an essential tool to study the role of pericytes in vascular development and regeneration and to better understand the therapeutic potential of miRNA manipulation in pericytes. Here we describe methods for manipulating pericyte function by using miRNA mimics and inhibitors. We also describe methods to assess pericyte function (proliferation and migration) after manipulation with miRNAs and explain how miRNA gene targets can be identified and validated in pericytes after manipulation with miRNA.

Keywords: Migration; Pericytes; Proliferation; Target validation; Transfection; miRNA inhibitors; miRNA mimics.

PubMed Disclaimer

References

    1. Ambros V (2004) The functions of animal microRNAs. Nature 431(7006):350–355 - DOI
    1. Asli NS, Pitulescu ME, Kessel M (2008) MicroRNAs in organogenesis and disease. Curr Mol Med 8(8):698–710 - DOI
    1. Larsson E et al (2009) Discovery of microvascular miRNAs using public gene expression data: miR-145 is expressed in pericytes and is a regulator of Fli1. Genome Med 1(11):108 - DOI
    1. Truettner JS et al (2013) Hypoxia alters MicroRNA expression in rat cortical pericytes. Microrna 2(1):32–44 - DOI
    1. Katare R et al (2011) Transplantation of human pericyte progenitor cells improves the repair of infarcted heart through activation of an angiogenic program involving micro-RNA-132. Circ Res 109(8):894–906 - DOI

Publication types

LinkOut - more resources