Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021:2235:155-167.
doi: 10.1007/978-1-0716-1056-5_11.

Isolation of Mammalian Mesoangioblasts: A Subset of Pericytes with Myogenic Potential

Giorgia Giacomazzi  1 , Gaia Giovannelli  1   2 , Alessio Rotini  1   2 , Domiziana Costamagna  1 , Mattia Quattrocelli  1 , Maurilio Sampaolesi  3   4
Affiliations

Isolation of Mammalian Mesoangioblasts: A Subset of Pericytes with Myogenic Potential

Giorgia Giacomazzi et al. Methods Mol Biol. 2021.

Abstract

Mesoangioblasts (MABs) are vessel-associated stem cells that express pericyte markers and are originally isolated from the embryonic dorsal aorta. From postnatal small vessels of skeletal muscle and heart, it is possible to isolate cells with similar characteristics to embryonic MABs. Adult MABs have the capacity to self-renew and to differentiate into cell types of mesodermal lineages upon proper culture conditions. To date, the origin of MABs and the relationship with other muscle stem cells are still debated. Recently, in a phase I-II clinical trial, intra-arterial HLA-matched MABs were proved to be relatively safe. Novel information on MAB pure populations is desirable, and implementation of their therapeutic potential is mandatory to approach efficacy in MAB-based treatments. This chapter provides an overview of the current techniques for isolation and characterization of rodent, canine, human, and equine adult MABs.

Keywords: Alkaline phosphatase; FACS; Mesoangioblast; Mesodermal lineage differentiation; Muscle stem cells.

PubMed Disclaimer

References

    1. Kuang S, Kuroda K, Le Grand F (2007) Rudnicki MA asymmetric self-renewal and commitment of satellite stem cells in muscle. Cell 129(5):999–1010 - DOI
    1. Cossu G, Biressi S (2005) Satellite cells, myoblasts and other occasional myogenic progenitors: possible origin, phenotypic features and role in muscle regeneration. Semin Cell Dev Biol 16(4-5):623–631 - DOI
    1. Dellavalle A, Sampaolesi M, Tonlorenzi R, Tagliafico E, Sacchetti B, Perani L et al (2007) Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cells. Nat Cell Biol 9(3):255–267 - DOI
    1. Dellavalle A, Maroli G, Covarello D, Azzoni E, Innocenzi A, Perani L et al (2011) Pericytes resident in postnatal skeletal muscle differentiate into muscle fibres and generate satellite cells. Nat Commun 2:499 - DOI
    1. Quattrocelli M, Costamagna D, Giacomazzi G, Camps J, Sampaolesi M (2014) Notch signaling regulates myogenic regenerative capacity of murine and human mesoangioblasts. Cell Death Dis 5:e1448 - DOI

Publication types

LinkOut - more resources