Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2021:2246:17-33.
doi: 10.1007/978-1-0716-1115-9_2.

FISH Variants

Nuno M Guimarães  1 , Nuno F Azevedo  2 , Carina Almeida  2   3   4
Affiliations
Review

FISH Variants

Nuno M Guimarães et al. Methods Mol Biol. 2021.

Abstract

FISH has gained an irreplaceable place in microbiology because of its ability to detect and locate a microorganism, or a group of organisms, within complex samples. However, FISH role has evolved drastically in the last few decades and its value has been boosted by several advances in signal intensity, imaging acquisitions, automation, method robustness, and, thus, versatility. This has resulted in a range of FISH variants that gave researchers the ability to access a variety of other valuable information such as complex population composition, metabolic activity, gene detection/quantification, or subcellular location of genetic elements. In this chapter, we will review the more relevant FISH variants, their intended use, and how they address particular challenges of classical FISH.

Keywords: CARD-FISH; CLASI-FISH; DOPE-FISH; FISH; FISH-NanoSIMS; Flow-FISH; Gene-FISH; Leaf-FISH; MAR-FISH; Microfluidic-FISH; NAM-FISH; Phage-FISH; QD-FISH.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Levsky JM, Singer RH (2003) Fluorescence in situ hybridization: past, present and future. J Cell Sci 116(Pt 14):2833–2838. https://doi.org/10.1242/jcs.00633 - DOI - PubMed
    1. Drescher J, Schlafer S, Schaudinn C, Riep B, Neumann K, Friedmann A, Petrich A, Gobel UB, Moter A (2010) Molecular epidemiology and spatial distribution of Selenomonas spp. in subgingival biofilms. Eur J Oral Sci 118(5):466–474. https://doi.org/10.1111/j.1600-0722.2010.00765.x - DOI - PubMed
    1. Frickmann H, Zautner AE, Moter A, Kikhney J, Hagen RM, Stender H, Poppert S (2017) Fluorescence in situ hybridization (FISH) in the microbiological diagnostic routine laboratory: a review. Crit Rev Microbiol 43(3):263–293. https://doi.org/10.3109/1040841X.2016.1169990 - DOI - PubMed
    1. Schonhuber W, Fuchs B, Juretschko S, Amann R (1997) Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification. Appl Environ Microbiol 63(8):3268–3273 - DOI
    1. Bobrow MN, Harris TD, Shaughnessy KJ, Litt GJ (1989) Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J Immunol Methods 125(1–2):279–285 - DOI

Publication types

MeSH terms

LinkOut - more resources