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. 2021 Apr;18(4):1058-1060.
doi: 10.1038/s41423-021-00641-8. Epub 2021 Feb 12.

Differential efficiencies to neutralize the novel mutants B.1.1.7 and 501Y.V2 by collected sera from convalescent COVID-19 patients and RBD nanoparticle-vaccinated rhesus macaques

Rong Li #  1   2   3   4 , Xiancai Ma #  1   2   3   4 , Jieyi Deng  1   2   3   4 , Qier Chen  1   2   3   4 , Weiwei Liu  1   2   3   4 , Zhilin Peng  1   2   3   4 , Yidan Qiao  1   2   3   4 , Yingtong Lin  1   2   3   4 , Xin He  5   6   7   8 , Hui Zhang  9   10   11   12
Affiliations

Differential efficiencies to neutralize the novel mutants B.1.1.7 and 501Y.V2 by collected sera from convalescent COVID-19 patients and RBD nanoparticle-vaccinated rhesus macaques

Rong Li et al. Cell Mol Immunol. 2021 Apr.
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Neutralizing potential of sera from convalescent patients and RBD nanoparticle-vaccinated rhesus macaques against SARS-CoV-2 variants. a Schematic of the spike protein of SARS-CoV-2 variants, including G614, D614, B.1.1.7, and 501Y.V2. Mutation types and positions are indicated next to each backbone. b Different pseudotyped viruses were incubated with serially diluted sera from convalescent COVID-19 patients and healthy controls to detect neutralizing antibodies. Neutralization potency was calculated by measuring the luciferase activity of infected hACE2-HeLa cells and plotted as a neutralization curve. Black lines represent the sera from healthy donors. c NT50 of each convalescent serum against each pseudotyped virus. d Different pseudotyped viruses were incubated with serially diluted sera from nanoparticle-vaccinated rhesus macaques to detect neutralizing antibodies. Neutralization potency was plotted as a neutralization curve. e NT50 of each serum from nanoparticle-vaccinated rhesus macaques against each pseudotyped virus. The NT50 titer in (c) and (e) was defined as the reciprocal of serum dilution at which nAbs caused 50% inhibition of infection. Data in (c) were analyzed by the Friedman test with Dunn’s multiple comparisons test (n = 49). ***p < 0.001. Data in (e) represented as the mean ± SEM (n = 4). Adjusted p values were calculated by two-way ANOVA with Tukey’s multiple comparisons test *p < 0.05
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