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Review
. 2019 Oct 25;49(1):9.
doi: 10.1186/s42649-019-0011-7.

Liquid electron microscopy: then, now and future

Anahita Vispi Bharda  1 , Hyun Suk Jung  2
Affiliations
Review

Liquid electron microscopy: then, now and future

Anahita Vispi Bharda et al. Appl Microsc. .

Abstract

Contemporary microscopic imaging at near-atomic resolution of diverse embodiments in liquid environment has gained keen interest. In particular, Electron Microscopy (EM) can provide comprehensive framework on the structural and functional characterization of samples in liquid phase. In the past few decades, liquid based electron microscopic modalities have developed tremendously to provide insights into various backgrounds like biological, chemical, nanoparticle and material researches. It serves to be a promising analytical tool in deciphering unique insights from solvated systems. Here, the basics of liquid electron microscopy with few examples of its applications are summarized in brief. The technical developments made so far and its preference over other approaches is shortly presented. Finally, the experimental limitations and an outlook on the future technical advancement for liquid EM have been discussed.

Keywords: Electron microscopy; Liquid-phase; Nanoparticle; STEM; TEM.

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Conflict of interest statement

The authors declare that they have no competing interests..

References

    1. Abrams IM, McBain JW. A closed cell for electron microscopy. Science. 1944;100:273–274. doi: 10.1126/science.100.2595.273. - DOI - PubMed
    1. Adrian M, Dubochet J, Lepault J, McDowall AW. Cryo-electron microscopy of viruses. Nature. 1984;308:32–36. doi: 10.1038/308032a0. - DOI - PubMed
    1. Ahmad N, Wang G, Nelayah J, Ricolleau C, Alloyeau D. Driving reversible redox reactions at solid-liquid interfaces with the electron beam of a transmission electron microscope. J. Microsc. 2018;269:127–133. doi: 10.1111/jmi.12568. - DOI - PubMed
    1. Arkhipov A, Shan Y, Das R, Endres NF, Eastwood MP, Wemmer DE, Kuriyan J, Shaw DE. Architecture and membrane interactions of the EGF Receptor. Cell. 2013;152:557–569. doi: 10.1016/j.cell.2012.12.030. - DOI - PMC - PubMed
    1. J.H. Au-Chang, J.Y. Au-Cheong, H.K. Au-Seo, I.-D. Au-Kim, J.M. Au-Yuk, Preparation of Graphene Liquid Cells for the Observation of Lithium-ion Battery Material. J Vis Exp (2019). 10.3791/58676 - PubMed

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