Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jan 15:11:573344.
doi: 10.3389/fgene.2020.573344. eCollection 2020.

Single-Nucleotide Polymorphism Variations Associated With Specific Genes Putatively Identified Enhanced Genetic Predisposition for 305-Day Milk Yield in the Girolando Crossbreed

Alex Silva da Cruz  1 , Danilo Conrado Silva  2 , Lysa Bernardes Minasi  1 , Larissa Kamídia de Farias Teixeira  1 , Flávia Melo Rodrigues  1 , Claudio Carlos da Silva  1 , Adriana Santana do Carmo  3 , Marcos Vinicius Gualberto Barbosa da Silva  4 , Yuri Tani Utsunomiya  5 , José Fernando Garcia  5 , Aparecido Divino da Cruz  1
Affiliations

Single-Nucleotide Polymorphism Variations Associated With Specific Genes Putatively Identified Enhanced Genetic Predisposition for 305-Day Milk Yield in the Girolando Crossbreed

Alex Silva da Cruz et al. Front Genet. .

Abstract

Milk production phenotypes are the main focus of genetic selection in dairy herds, and although there are many genes identified as related to the biology of these traits in pure breeds, little is known about crossbreed animals. This study aimed to identify potential genes associated with the 305-day milk yield in 337 crossbreed Gir × Holstein (Girolando) animals. Milk production records were genotyped for 45,613 single-nucleotide polymorphisms (SNPs). This dataset was used for a genome-wide association study (GWAS) using the 305-day milk yield adjusted for the fixed effects of herd and year and linear and quadratic effects of age at calving (in days) and calving factor averaged per animal. Genes within the significant SNPs were retrieved from the Bos taurus ARS-UCD1.2 assembly (bosTau9) for gene ontology analysis. In summary, the GWAS identified 52 SNPs associated [p ≤ 10-4, false discovery rate (FDR) = 8.77%] with milk production, including NUB1 and SLC24A2, which were previously described as related to milk production traits in cattle. The results suggest that SNPs associated mainly with NUB1 and SLC24A2 could be useful to understand milk production in Girolando and used as predictive markers for selecting genetic predisposition for milk yield in Girolando.

Keywords: GWAS; SNP; dairy cattle; indicine; selection.

PubMed Disclaimer

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Manhattan plot displaying the results of the genome-wide analysis of milk production adjusted to 305 days in Girolando cattle.
See this image and copyright information in PMC

References

    1. Akey D. T., Zhu X., Dyer M., Li A., Sorensen A., Blackshaw S., et al. (2002). The inherited blindness associated protein AIPL1 interacts with the cell cycle regulator protein NUB1. Hum. Mol. Genet. 11 2723–2733. 10.1093/hmg/11.22.2723 - DOI - PMC - PubMed
    1. Altimimi H. F., Fung E. H., Winkfein R. J., Schnetkamp P. P. M. (2010). Residues contributing to the Na+-binding pocket of the SLC24 Na+/Ca2+-K+ exchanger NCKX2. J. Biol. Chem. 285 15245–15255. 10.1074/jbc.M109.090738 - DOI - PMC - PubMed
    1. Arcot S. K., Battista R., Keep R. B., Capasso J. E., Levin A. V. (2015). Autosomal-dominant leber congenital amaurosis caused by a heterozygous CRX mutation in a father, and son. Ophthalmic Genet. 36 156–159. 10.3109/13816810.2013.838273 - DOI - PubMed
    1. Aslibekyan S., Kabagambe E. K., Irvin M. R., Straka R. J., Borecki I. B., Tiwari H. K., et al. (2012). A genome-wide association study of inflammatory biomarker changes in response to fenofibrate treatment in the genetics of lipid lowering drug and diet network: pharmacogenet. Genomics 22 191–197. 10.1097/FPC.0b013e32834fdd41 - DOI - PMC - PubMed
    1. Associação Brasileira de Criadores de Bovinos da Raça Holandesa [ABCBRH] (1986). Regimento do Serviço de Controle Leiteiro, Vol. 1 São Paulo, 1–5.

LinkOut - more resources