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Review
. 2021 Jan 27:11:610386.
doi: 10.3389/fgene.2020.610386. eCollection 2020.

Emerging Technologies for Genome-Wide Profiling of DNA Breakage

Matthew J Rybin  1   2 , Melina Ramic  1   2 , Natalie R Ricciardi  1   2 , Philipp Kapranov  3 , Claes Wahlestedt  1   2 , Zane Zeier  1   2
Affiliations
Review

Emerging Technologies for Genome-Wide Profiling of DNA Breakage

Matthew J Rybin et al. Front Genet. .

Abstract

Genome instability is associated with myriad human diseases and is a well-known feature of both cancer and neurodegenerative disease. Until recently, the ability to assess DNA damage-the principal driver of genome instability-was limited to relatively imprecise methods or restricted to studying predefined genomic regions. Recently, new techniques for detecting DNA double strand breaks (DSBs) and single strand breaks (SSBs) with next-generation sequencing on a genome-wide scale with single nucleotide resolution have emerged. With these new tools, efforts are underway to define the "breakome" in normal aging and disease. Here, we compare the relative strengths and weaknesses of these technologies and their potential application to studying neurodegenerative diseases.

Keywords: DNA damage; aging; double strand break (DSB); genome instability; neurodegeneration; neurodegenerative disease; single strand break (SSB).

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
General workflow for genome-wide, nucleotide-resolution detection of DSBs. Created with BioRender.com.
Figure 2
Figure 2
Comparison of genome-wide, nucleotide-resolution SSB mapping techniques. Created with BioRender.com.
See this image and copyright information in PMC

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