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. 2020 Winter;13(Suppl1):S60-S67.

Evaluation of expression of common genes in the intestine and peripheral blood mononuclear cells (PBMC) associated with celiac disease

Ensieh Khalkhal  1 , Fatemeh Nobakht  2 , Mohammad Hossain Haidari  1 , Zahra Razaghi  3 , Mahsa Ghasemzad  4 , Melika Sheikhan  4 , Mohammad Rostami Nejad  5
Affiliations

Evaluation of expression of common genes in the intestine and peripheral blood mononuclear cells (PBMC) associated with celiac disease

Ensieh Khalkhal et al. Gastroenterol Hepatol Bed Bench. 2020 Winter.

Abstract

Aim: this study was conducted to investigate expression of the genes associated with CD in the target tissue in order to estimate contribution of each single gene to development of immune response. Then, the same set of genes was evaluated in peripheral blood mononuclear cells (PBMCs).

Background: Celiac disease (CD) is a chronic systemic autoimmune disease of the small intestine occurring in genetically-susceptible individuals. There are several genes related to immune response.

Methods: For this purpose, the genes related to CD were extracted from public databases (documents of proteomics and microarray-based techniques) and were organized in a protein-protein interaction network using the search tool for retrieval of interacting genes/proteins (STRING) database as a plugin of Cytoscape software version 3.6.0. The main genes were introduced and enriched via ClueGO to find the related biochemical pathways. The network was analyzed, and the most important genes were introduced based on central indices.

Results: Among 20 CD genes as hub and bottleneck nodes, there were 7 genes with common expression in blood and intestinal tissue (C-X-C motif chemokine 11(CXCL11), granzyme B (GZMB), interleukin 15(IL-15), interleukin 17(IL-17A), interleukin 23(IL-23A), t-box transcription factor 21(TBX21), and tumor necrosis factor alpha-induced protein 3(TNFAIP3)).

Conclusion: The enriched biological process related to the central nodes of celiac network indicated that most of hub-bottleneck genes are the well-known ones involved in different types of autoimmune and inflammatory diseases.

Keywords: Autoimmune; Celiac Disease; Inflammatory; Intestinal Tissue; PBMC.

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Figures

Figure 1
Figure 1
PPI network of CD constructed by 533 genes extracted from STRING database
Figure 2
Figure 2
The 20 central nodes of CD network organized in a sub-network
See this image and copyright information in PMC

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