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. 2021 Mar;106(3):e21766.
doi: 10.1002/arch.21766. Epub 2021 Feb 15.

Three endogenous cellulases from termite, Reticulitermes speratus KMT001

Hee-Hoon Ahn  1 , Tae-Jong Kim  1
Affiliations

Three endogenous cellulases from termite, Reticulitermes speratus KMT001

Hee-Hoon Ahn et al. Arch Insect Biochem Physiol. 2021 Mar.

Abstract

Among termites, lower termites need symbiotic microorganisms in the digestive tract for digestion and cellulose metabolism. In this symbiotic relationship, the decomposition of cellulose is initiated by endoglucanase in termite salivary glands and completed by β-glycosidase of symbiotic microorganisms in the hindgut. The expression of β-glycosidase in lower termites has been reported in recent studies. The expression of two endoglucanases and one β-glycosidase gene related to cellulose degradation was identified in Reticulitermes speratus, a lower termite, through transcriptomic analysis. The proposed enzyme activities of three identified cellulose degradation genes were confirmed by heterologous expression in Escherichia coli. In addition to the endoglucanase expressed in the salivary gland, additional endoglucanase and β-glycosidase genes suggest that R. speratus performs the overall cellulose digestion using its own enzymes at all stages.

Keywords: BLASTp; Reticulitermes speratus; cellulose; digestive tract; endoglucanase; termite; β-glycosidase.

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References

REFERENCES

    1. Argyropoulos, D. S. (2001). Wood and cellulosic chemistry. Second edition. Journal of the American Chemical Society, 123(36), 8880-8881. https://doi.org/10.1021/ja015237p
    1. Cho, M.-J., Kim, Y.-H., Shin, K., Kim, Y.-K., Kim, Y.-S., & Kim, T.-J. (2010). Symbiotic adaptation of bacteria in the gut of Reticulitermes speratus: Low endo-β-1,4-glucanase activity. Biochemical and Biophysical Research Communications, 395(3), 432-435. https://doi.org/10.1016/j.bbrc.2010.04.048
    1. Cho, M.-J., Shin, K., Kim, Y.-K., Kim, Y.-S., & Kim, T.-J. (2010). Phylogenetic analysis of Reticulitermes speratus using the mitochondrial cytochrome C oxidase subunit I gene. Journal of the Korean Wood Science and Technology, 38(2), 135-139. https://doi.org/10.5658/wood.2010.38.2.135
    1. Cleveland, L. R. (1924). The physiological and symbiotic relationships between the intestinal protozoa of termites and their host, with special reference to Reticulitermes flavipes Kollar. Biological Bulletin, 46(4), 178-201.
    1. Davison, A., & Blaxter, M. (2005). Ancient origin of glycosyl hydrolase family 9 cellulase genes. Molecular Biology and Evolution, 22(5), 1273-1284. https://doi.org/10.1093/molbev/msi107

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