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. 2021 Aug;40(8):1761-1766.
doi: 10.1007/s10096-021-04198-2. Epub 2021 Feb 17.

KPC-12 with a L169M substitution in the Ω loop has reduced carbapenemase activity

Jiayuan Qin  1   2 , Yu Feng  1   2   3 , Xiaoju Lü  1   2 , Zhiyong Zong  4   5   6   7
Affiliations

KPC-12 with a L169M substitution in the Ω loop has reduced carbapenemase activity

Jiayuan Qin et al. Eur J Clin Microbiol Infect Dis. 2021 Aug.

Abstract

KPC-12 is a variant of KPC-2 with a L169M substitution in the Ω loop, but its resistance spectrum was not reported. blaKPC-12 was cloned, and KPC-12 exhibited significantly decreased activities against imipenem, meropenem, aztreonam, and piperacillin-tazobactam with ≥4-fold lower MICs than KPC-2. However, unlike the L169P substitution in KPC-35, activities against ceftazidime and ceftazidime-avibactam of KPC-12 were unaltered. This highlights that different substitutions at the same position of carbapenemases may have varied impact on the activity.

Keywords: Carbapenem resistance; Carbapenemase; KPC-12; Omega loop.

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References

    1. Yigit H, Queenan AM, Anderson GJ, Domenech-Sanchez A, Biddle JW, Steward CD, Alberti S, Bush K, Tenover FC (2001) Novel carbapenem-hydrolyzing β-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae. Antimicrob Agents Chemother 45:1151–1161 - DOI
    1. Giddins MJ, Macesic N, Annavajhala MK, Stump S, Khan S, McConville TH, Mehta M, Gomez-Simmonds A, Uhlemann AC (2018) Successive emergence of ceftazidime-avibactam resistance through distinct genomic adaptations in blaKPC-2-harboring Klebsiella pneumoniae sequence type 307 isolates. Antimicrob Agents Chemother 62:e02101–e02117 - DOI
    1. Winkler ML, Papp-Wallace KM, Bonomo RA (2015) Activity of ceftazidime/avibactam against isogenic strains of Escherichia coli containing KPC and SHV β-lactamases with single amino acid substitutions in the Ω-loop. J Antimicrob Chemother 70:2279–2286 - DOI
    1. Hemarajata P, Humphries RM (2019) Ceftazidime/avibactam resistance associated with L169P mutation in the omega loop of KPC-2. J Antimicrob Chemother 74:1241–1243 - DOI
    1. Tamma PD, Aitken SL, Bonomo RA, Mathers AJ, van Duin D, Clancy CJ (2020) Infectious Diseases Society of America Antimicrobial Resistant Treatment Guidance: gram-negative bacterial infections. Clin Infect Dis. https://doi.org/10.1093/cid/ciaa1478

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