Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jun;106(5):1219-1232.
doi: 10.1111/tpj.15204. Epub 2021 May 15.

NOL-mediated functional stay-green traits in perennial ryegrass (Lolium perenne L.) involving multifaceted molecular factors and metabolic pathways regulating leaf senescence

Guohui Yu  1   2 , Zheni Xie  1 , Jing Zhang  1 , Shanshan Lei  1 , Wenjing Lin  1 , Bin Xu  1 , Bingru Huang  2
Affiliations
Free article

NOL-mediated functional stay-green traits in perennial ryegrass (Lolium perenne L.) involving multifaceted molecular factors and metabolic pathways regulating leaf senescence

Guohui Yu et al. Plant J. 2021 Jun.
Free article

Abstract

Loss of chlorophyll (Chl) is a hallmark of leaf senescence, which may be regulated by Chl catabolic genes, including NON-YELLOW COLORING 1 (NYC1)-like (NOL). The objective of this study was to determine molecular factors and metabolic pathways underlying NOL regulation of leaf senescence in perennial grass species. LpNOL was cloned from perennial ryegrass (Lolium perenne L.) and found to be highly expressed in senescent leaves. Transient overexpression of LpNOL accelerated leaf senescence and Chl b degradation in Nicotiana benthamiana. LpNOL RNA interference (NOLi) in perennial ryegrass not only significantly blocked Chl degradation in senescent leaves, but also delayed initiation and progression of leaf senescence. This study found that NOL, in addition to functioning as a Chl b reductase, could enact the functional stay-green phenotype in perennial grass species, as manifested by increased photosynthetic activities in NOLi plants. Comparative transcriptomic analysis revealed that NOL-mediated functional stay-green in perennial ryegrass was mainly achieved through the modulation of Chl catabolism, light harvesting for photosynthesis, photorespiration, cytochrome respiration, carbohydrate catabolism, oxidative detoxification, and abscisic acid biosynthesis and signaling pathways.

Keywords: NYC1-LIKE (NOL); chlorophyll; photosynthesis; ryegrass; senescence; stay-green; transcriptome.

PubMed Disclaimer

References

REFERENCES

    1. Allen, J.F. & Forsberg, J. (2001) Molecular recognition in thylakoid structure and function. Trends in Plant Science, 6, 317-326.
    1. Armstead, I., Donnison, I., Aubry, S., Harper, J., Hörtensteiner, S., James, C. et al. (2006) From crop to model to crop: identifying the genetic basis of the staygreen mutation in the Lolium/Festuca forage and amenity grasses. New Phytologist, 172, 592-597.
    1. Arnon, D.I. (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24, 1-15.
    1. Arriola, K.G., Kim, S.C., Huisden, C.M. & Adesogan, A.T. (2012) Stay-green ranking and maturity of corn hybrids: 1. Effects on dry matter yield, nutritional value, fermentation characteristics, and aerobic stability of silage hybrids in Florida. Journal of Dairy Science, 95, 964-974.
    1. Bailey, S., Walters, R.G., Jansson, S. & Horton, P. (2001) Acclimation of Arabidopsis thaliana to the light environment: the existence of separate low light and high light responses. Planta, 213, 794-801.

Publication types

MeSH terms

LinkOut - more resources