Impacts of agricultural practices on pyrethroid resistance in Culex pipiens pipiens, an important vector of human diseases, from Tunisia

Abstract

Agricultural pesticides may play a profound role in selection of resistance in field populations of mosquito vectors. The aim of the present study was to examine the relationship between agricultural pesticide use and development of resistance to insecticides in Culex pipiens pipiens from Tunisia. Entomological surveys were conducted in three various districts from Tunisia differ in insect control in agriculture and in public health. A reference locality without any chemical activities was used to do different comparisons. Our results revealed that the level of permethrin resistance ranged from 40.9 to 7438. Practically no susceptible populations were found and resistance to permethrin was important, but significantly higher in site submitted to both agricultural and public health applications. However, resistance ratio has been decreased 7000 folds in site not submitted to agricultural pests. These observations expressed an important influence of agricultural applications on permethrin resistance and need an urgent coordination between the integrated vector control program and the Ministry of Agriculture to reduce the development of resistance in populations. The recorded resistance was slightly associated with DDT suggest the involvement of their common mechanism (target site). Synergist's tests indicated that different enzymes played an important role in the detoxification of this insecticide.