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. 2021 Feb 5;2(1):100331.
doi: 10.1016/j.xpro.2021.100331. eCollection 2021 Mar 19.

Monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection

Yaarit Adamovich  1 , Saar Ezagouri  1 , Vaishnavi Dandavate  1 , Gad Asher  1
Affiliations

Monitoring daytime differences in moderate intensity exercise capacity using treadmill test and muscle dissection

Yaarit Adamovich et al. STAR Protoc. .

Abstract

There is growing interest in medicine and sports in uncovering exercise modifiers that enhance or limit exercise capacity. Here, we detail a protocol for testing the daytime effect on running capacity in mice using a moderate intensity treadmill effort test. Instructions for dissecting soleus, gastrocnemius plantaris, and quadriceps muscles for further analysis are provided as well. This experimental setup is optimized for addressing questions regarding the involvement of daytime and circadian clocks in regulating exercise capacity. For complete details on the use and execution of this protocol, please refer to Ezagouri et al. (2019).

Keywords: Metabolism; Model organisms.

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Conflict of interest statement

The authors declare no competing interests.

Figures

None
Graphical abstract
Figure 1
Figure 1
An illustration of ZT coordinates in conjunction with rest-active phases and light-dark schedule
Figure 2
Figure 2
An illustration of using different light-dark schedule to allow performing the protocol at two different ZTs simultaneously
Figure 3
Figure 3
An example of glucose monitoring from a mouse tail tip
Figure 4
Figure 4
Expected blood glucose levels during moderate intensity (25 cm/s) running test performed at two different ZTs during the active phase Data are presented as the mean ± SEM of n = 3. p < 0.05, p < 0.01, p < 0.001 repeated measures two-way ANOVA for the interaction, ZT effect, and running duration effect, respectively.
Figure 5
Figure 5
Dissection of mouse soleus (Sol), gastrocnemius plantaris (Gas), and quadriceps (Quad) muscles
See this image and copyright information in PMC

References

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