Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977;55(1-2):107-19.
doi: 10.1007/BF01314484.

The rapid concentration and purification of influenza virus from allantoic fluid

J T Heyward, R A Klimas, M D Stapp, J F Obijeski

The rapid concentration and purification of influenza virus from allantoic fluid

J T Heyward et al. Arch Virol. 1977.

Abstract

Influenza virus was quantitatively recovered from infectious allantoic fluid by precipitation with 8 percent (wt/vol) polyethylene glycol 6000 (PEG-6000). Virus concentrated by PEG-6000 treatment was rapidly purified by gel permeation chromatography through controlled pore glass beads. The purity of virus preparations achieved by this method, when judged by three independent criteria, was comparable to virus preparations purified by conventional density gradient procedures.

PubMed Disclaimer

References

    1. Biochim Biophys Acta. 1969 Oct 22;190(2):460-9 - PubMed
    1. J Chromatogr. 1973 Oct 10;85(1):129-31 - PubMed
    1. J Gen Virol. 1974 Dec;25(3):351-7 - PubMed
    1. Bull World Health Organ. 1961;24:695-702 - PubMed
    1. Acta Virol. 1972 Jun;16(1):53-62 - PubMed

LinkOut - more resources