California encephalitis virus transmission by arctic and domestic mosquitoes

Abstract

A zero passage arctic mosquito isolate of California encephalitis (CE) virus (showshoe hare subtype) was transmitted by wild-caught Aedes communis mosquitoes after 13 days incubation at 13 degrees and 23 degrees C, AFTER 20 days incubation at 13 degrees C, when mosquitoes imbibed 1 mouse LD50 in a blood meal. Transmission occurred after 20 days incubation at 13 degrees and 23 degrees C when mosquitoes were injected intrathoracially with 1 or 0.1 mouse LD50. Virus was also transmitted by A. aegypti 13 days after infection with 100 mouse LD50 by feeding or intrathoracic injection, and incubation at 13 degrees C. Virus antigen was detected in salivary glands of 42 percent virus-positive A communis mosquitoes by direct immunofluorescence, and in 50 percent or less of A aegypti mosquitoes by immunoperoxidase and immunofluorescence, with somewhat greater regularity by the indirect than the direct technique.