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. 1988 Apr 11;231(1):225-8.
doi: 10.1016/0014-5793(88)80736-1.

Biosyntheses and processing of lysosomal cysteine proteinases in rat macrophages

E Kominami  1 , T Tsukahara, K Hara, N Katunuma
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Free article

Biosyntheses and processing of lysosomal cysteine proteinases in rat macrophages

E Kominami et al. FEBS Lett. .
Free article

Abstract

The intracellular processing and release of three lysosomal cysteine proteinases, cathepsin B, H and L, by rat peritoneal macrophages were investigated by pulse-chase experiments. Newly synthesized procathepsins B (39 kDa), H(41 kDa) and L (39 kDa) after 15 min labeling were processed to the mature, single-chain enzymes within 1 h. The single-chain forms of cathepsin B, H and L were further processed to two-chain forms at different rates: conversion of cathepsin L to the two-chain form was rapid, whereas the conversions cathepsin B and H took at least 6 h. Macrophages released 30% of the procathepsins B and L, and 10% of the procathepsin H.

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