Measurement of the Size and Concentration and Zeta Potential of Extracellular Vesicles Using Nanoparticle Tracking Analyzer

doi:10.1007/978-1-0716-1246-0_15

. 2021:2273:207-218.

doi: 10.1007/978-1-0716-1246-0_15.

Measurement of the Size and Concentration and Zeta Potential of Extracellular Vesicles Using Nanoparticle Tracking Analyzer

Keerthie Dissanayake¹, Getnet Midekessa¹, Freddy Lättekivi¹, Alireza Fazeli^{2

3}

Affiliations

¹ Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
² Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia. alireza.fazeli@ut.ee.
³ Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK. alireza.fazeli@ut.ee.

PMID: 33604856
DOI: 10.1007/978-1-0716-1246-0_15

Measurement of the Size and Concentration and Zeta Potential of Extracellular Vesicles Using Nanoparticle Tracking Analyzer

Keerthie Dissanayake et al. Methods Mol Biol. 2021.

. 2021:2273:207-218.

doi: 10.1007/978-1-0716-1246-0_15.

Authors

Keerthie Dissanayake¹, Getnet Midekessa¹, Freddy Lättekivi¹, Alireza Fazeli^{2

3}

Affiliations

¹ Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
² Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia. alireza.fazeli@ut.ee.
³ Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK. alireza.fazeli@ut.ee.

PMID: 33604856
DOI: 10.1007/978-1-0716-1246-0_15

Abstract

Extracellular vesicles (EVs) are membrane-bound nanoparticles that are secreted by most cell types with an emerging role in cellular communication and potential as biomarkers of disease. Nanoparticle tracking analysis (NTA) is a commonly used technique to measure the size and concentration of nanoparticles, such as EVs. Here, we present two protocols for the analysis of size profile concentration, and zeta potential (ZP) of well-characterized EVs derived from human choriocarcinoma JAr cells using NTA. These protocols describe how the size profile concentration, and ZP of JAr EVs are measured using optimized settings of NTA. With good experimental practices and consistent protocol, NTA measurements of EVs can provide reliable data that could potentially translate further uses of EVs for diagnostic and therapeutic applications.

Keywords: Extracellular vesicles; Nanoparticle tracking analysis; Size profile; Zeta potential.

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Characterization of Extracellular Vesicles Labelled with a Lipophilic Dye Using Fluorescence Nanoparticle Tracking Analysis.
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References

1. Raposo G, Stoorvogel W (2013) Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol 200(4):373–383 - DOI
1. Théry C, Witwer KW, Aikawa E et al (2019) Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles 7:1535750 - DOI
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1. Midekessa G et al (2020) Zeta potential of extracellular vesicles: toward understanding the attributes that determine colloidal stability. ACS Omega 5(27):16701–16710 - DOI

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[1] Raposo G, Stoorvogel W (2013) Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol 200(4):373–383 - DOI

[2] Raposo G, Stoorvogel W (2013) Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol 200(4):373–383 - DOI

[3] Théry C, Witwer KW, Aikawa E et al (2019) Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles 7:1535750 - DOI

[4] Théry C, Witwer KW, Aikawa E et al (2019) Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles 7:1535750 - DOI

[5] Gardiner C, Ferreira YJ, Dragovic RA et al (2013) Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis. J Extracell Vesicles 2:19671 - DOI

[6] Gardiner C, Ferreira YJ, Dragovic RA et al (2013) Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis. J Extracell Vesicles 2:19671 - DOI

[7] Thane KE, Davis AM, Hoffman AM (2019) Improved methods for fluorescent labeling and detection of single extracellular vesicles using nanoparticle tracking analysis. Sci Rep 9:12295 - DOI

[8] Thane KE, Davis AM, Hoffman AM (2019) Improved methods for fluorescent labeling and detection of single extracellular vesicles using nanoparticle tracking analysis. Sci Rep 9:12295 - DOI

[9] Midekessa G et al (2020) Zeta potential of extracellular vesicles: toward understanding the attributes that determine colloidal stability. ACS Omega 5(27):16701–16710 - DOI

[10] Midekessa G et al (2020) Zeta potential of extracellular vesicles: toward understanding the attributes that determine colloidal stability. ACS Omega 5(27):16701–16710 - DOI

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Measurement of the Size and Concentration and Zeta Potential of Extracellular Vesicles Using Nanoparticle Tracking Analyzer

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