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Comment
. 2021 Feb 18;81(4):647-648.
doi: 10.1016/j.molcel.2021.01.036.

CRISPR base editor screens identify variant function at scale

Phoebe C R Parrish  1 , Alice H Berger  2
Affiliations
Comment

CRISPR base editor screens identify variant function at scale

Phoebe C R Parrish et al. Mol Cell. .

Abstract

Cuella-Martin et al. (2021) and Hanna et al. (2021) showcase CRISPR base editing in large-scale pooled screens in human cells to discover both loss- and gain-of-function variants, enabling protein structure/function insights and clinical variant interpretation.

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Figures

Figure 1.
Figure 1.. Overview of CRISPR base editor screens.
(A) Two new studies perform CRISPR base editor screens using a cytidine deaminase/Cas9 nickase base editor that generates C>T mutations in target genes. (B) The authors designed sgRNA libraries to generate mutations at target sites in genes of interest, which were introduced into haploid and diploid human cell lines along with the base editor in viability and drug screens. The functional effects of selected mutations were validated to provide insight into protein structure and function, and to predict whether the variants were benign or pathogenic in a clinical context. Figure created using BioRender.com.
See this image and copyright information in PMC

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References

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