[Sequential Edman degredation]

Abstract

Since Edman's first publication in 1950, the stepwise degradation of proteins and peptides is universally performed by protein chemists. We extensively reviewed the different manual degradations. We take two examples of manual degradation: a semi-micromethod and a micromethod in order to illustrate the evolution of manual degradation. The "dansyl-Edman" procedure proposed by Hartley in 1963 completes the manual N-terminal determination of peptides. We describe the different procedures of identification of PTH-amino acids: paper chromatography, thin layer chromatography, gas chromatography and liquid chromatography under high pressure and various modified Edman degradation procedures. Possibilities and limits of the liquid phase Sequenator of Edman reported in 1967 and the solid phase Sequencer of Laursen reported in 1971 are also considered in detail.