Study on the correlation between Helicobacter Pylori and biological characteristics of early Gastric Cancer

Abstract

Objective: Retrospective analysis was used to determine the population diagnosed with EGC, and HP infection was used as the cut-off point to further evaluate the correlation between helicobacter pylori (HP) infection and tumor biological characteristics of early gastric cancer (EGC). Methods: All cases were collected from patients diagnosed with EGC through endoscopic surgery or surgical procedures from January 2009 to October 2018. General information, tumor site, tumor pathology, HER2 immunohistochemical results, and degree of HP infection were collected for retrospective analysis. Results: A total of 111 cases were collected in this study. Among the HP negative group, there were statistically significant differences in tumor sites between the uninfected group and the previously infected group (P<0.05).There were significant differences in monocyte infiltration and neutrophil infiltration between the positive and negative groups (P<0.05).The differentiated adenocarcinoma in the positive group was significantly lower than that in the negative group. The incidence rate of Mixed type cancer was significantly higher than that in the positive group (P<0.01). In the positive group of HP, there was a statistically significant difference in HER2 between the unsterilized group and the previously sterilized group (P<0.05).There was a statistically significant difference in HER2 between the HP positive group and the HP negative group (P<0.01). HP infection was significantly correlated with HER2 index and presented a positive correlation (P=0.014). Conclusion: HP infection is related to the tumor site and mucosal inflammatory infiltration of EGC. The malignant degree of EGC complicated with HP infection is higher, and most of them are mixed type. The degree of HP infection was positively correlated with the degree of invasion and malignancy of ECG. Furthermore, the tumor indicator HER2 is closely related to HP infection, and the detection of HP combined with HER2 is of great significance in the discovery of EGC and the evaluation of its malignancy.

Keywords: biological characteristics; early gastric cancer; helicobacter pylori.

Figure 1

The relationship between HP infection and early gastric cancer differentiation. The histological specimens were all stained with H-E, and the magnification is 100 times. A: HP (-), from uninfected group, differentiated type, highly differentiated adenocarcinoma, infiltrate into the mucosa; B: HP (2+), from unsterilized group, mixed type, moderately poorly differentiated adenocarcinoma and mucinous adenocarcinoma, infiltrate into the submucosa; C: HP (2+), from previously sterilized group, differentiated type, moderately differentiated adenocarcinoma, infiltrate into the submucosa; D: HP (3+), from previously sterilized group, differentiated type, moderately differentiated adenocarcinoma, infiltrate into the submucosa.

Figure 2

Immune-histochemical results of HER2 in different cases of HP infection. The brown-yellow particles in the figure represent the expression of HER2. A: HER2 negative (1+), from uninfected group, HP(-); B:HER2 positive (2+), from unsterilized group, HP(1+); C: HER2 negative (1+), from previously sterilized group, HP (2+); D: HER2 positive (3+), from unsterilized group, HP (2+); E: HER2 negative (1+), from previously sterilized group, HP (3+); F: HER2 positive (3+), from unsterilized group, HP (3+).