Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Mar;69(2):913-918.
doi: 10.1111/tbed.14042. Epub 2021 Apr 27.

A serological survey and characterization of Getah virus in domestic pigs in Thailand, 2017-2018

Khwankamon Rattanatumhi  1 , Noppadol Prasertsincharoen  1 , Nattakarn Naimon  1 , Ryusei Kuwata  2   3 , Hiroshi Shimoda  2 , Keita Ishijima  4 , Kenzo Yonemitsu  2 , Shohei Minami  2 , Supriyono  2 , Ngo Thuy Bao Tran  2 , Yudai Kuroda  2   4 , Kango Tatemoto  2   4 , Milagros Virhuez Mendoza  2   4 , Eiichi Hondo  5 , Worawut Rerkamnuaychoke  6 , Ken Maeda  2   4 , Thanmaporn Phichitraslip  1
Affiliations

A serological survey and characterization of Getah virus in domestic pigs in Thailand, 2017-2018

Khwankamon Rattanatumhi et al. Transbound Emerg Dis. 2022 Mar.

Abstract

Getah virus (GETV) is a mosquito-borne RNA virus belonging to the family Togaviridae, genus Alphavirus. GETV infection causes diarrhoea and death in piglets, and reproductive failure and abortion in sows. This study conducted a serological survey of GETV infection among domestic pig populations in Thailand. ELISA was used to analyse 1,188 pig serum samples collected from 11 provinces of Thailand during 2017-2018, with 23.1% of the samples being positive for anti-GETV antibodies. The positive ratio of anti-GETV antibodies was significantly higher in nursery (67.9%) and older stages (84.5%) of pigs than in finishing stage (14.2%). Furthermore, we successfully isolated GETV from one pig serum, designated as GETV strain GETV/SW/Thailand/2017, and determined the complete genome sequence (11,689 nt). Phylogenetic analysis demonstrated that our isolate was different from the recent GETV group spreading among pig populations in East Asia and formed a cluster with two GETV strains, namely YN12031 (China, 2015) and LEIV16275Mar (Far-East Russia, 2007). We concluded that two different GETV groups are currently spreading among pig populations in Asian countries.

Keywords: ELISA; Getah virus; Thailand; pig; serology; virus isolation.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

REFERENCES

    1. Bannai, H., Nemoto, M., Ochi, A., Kikuchi, T., Kobayashi, M., Tsujimura, K., & Kondo, T. (2015). Epizootiological investigation of Getah virus infection among racehorses in Japan in 2014. Journal of Clinical Microbiology, 53(7), 2286-2291. https://doi.org/10.1128/JCM.00550-15
    1. Chumakov, M. P., Moshkin, A. V., & Andreeva, E. B. (1974). Isolation of five Getah virus strains from mosquitoes in the southern Amur region, USSR (Russian). Trudy Instituta Poliomielita I Virusnykh Entsefalitov Akademii Meditsinskikh Nauk SSSR, 22(2), 65-71.
    1. Eshoo, M. W., Whitehouse, C. A., Zoll, S. T., Massire, C., Pennella, T.-T.- D., Blyn, L. B., & Ecker, D. J. (2007). Direct broad-range detection of alphaviruses in mosquito extracts. Virology, 368(2), 286-295. https://doi.org/10.1016/j.virol.2007.06.016
    1. Feng, Y., Fu, S., Zhang, H., Li, M., Zhou, T., Wang, J., & Liang, G. (2012). Distribution of mosquitoes and mosquito-borne viruses along the China-Myanmar border in Yunnan Province. Japanese Journal of Infectious Diseases, 65(3), 215-221. https://doi.org/10.7883/yoken.65.215
    1. Firth, A. E., Wills, N. M., Gesteland, R. F., & Atkins, J. F. (2011). Stimulation of stop codon readthrough: Frequent presence of an extended 3' RNA structural element. Nucleic Acids Research, 39(15), 6679-6691. https://doi.org/10.1093/nar/gkr224

LinkOut - more resources