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. 2021 Mar;18(3):249-252.
doi: 10.1038/s41592-021-01075-w. Epub 2021 Feb 22.

Genome-scale deconvolution of RNA structure ensembles

Edoardo Morandi #  1   2 , Ilaria Manfredonia #  1 , Lisa M Simon #  2 , Francesca Anselmi  2 , Martijn J van Hemert  3 , Salvatore Oliviero  4   5 , Danny Incarnato  6
Affiliations
Free article

Genome-scale deconvolution of RNA structure ensembles

Edoardo Morandi et al. Nat Methods. 2021 Mar.
Free article

Abstract

RNA structure heterogeneity is a major challenge when querying RNA structures with chemical probing. We introduce DRACO, an algorithm for the deconvolution of coexisting RNA conformations from mutational profiling experiments. Analysis of the SARS-CoV-2 genome using dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) and DRACO, identifies multiple regions that fold into two mutually exclusive conformations, including a conserved structural switch in the 3' untranslated region. This work may open the way to dissecting the heterogeneity of the RNA structurome.

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