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Editorial
. 2021 May-Jun;47(3):617-626.
doi: 10.1590/S1677-5538.IBJU.2019.0362.

Dysplasia of the fibrous sheath with axonemal and centriolar defects combined with lack of mitochondrial activity as associated factors of ICSI failure in primary ciliary dyskinesia syndrome

Juliana R Pariz  1   2   3 , Caroline Rané  1   2 , Joel Drevet  4 , Jorge Hallak  1   2   3   5
Affiliations
Editorial

Dysplasia of the fibrous sheath with axonemal and centriolar defects combined with lack of mitochondrial activity as associated factors of ICSI failure in primary ciliary dyskinesia syndrome

Juliana R Pariz et al. Int Braz J Urol. 2021 May-Jun.
No abstract available

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Conflict of interest statement

None declared.

Figures

Figure 1
Figure 1. Sperm morphology and mitochondrial activity representative photographs. A) Normal human sperm morphology according to WHO criteria is defined as follows: sperm head should be smooth, regularly contoured and generally oval in shape, a well-defined acrosomal region comprising 40-70% of the head area should be seen, the midpiece should be slender, regular and about the same length as the sperm head, the major axis of the midpiece should be aligned with the major axis of the sperm head. Residual cytoplasm is considered an anomaly only when in excess, the principal piece should have a uniform caliber along its length (45µm long, approximately 10 times the head legth) and be thinner than the midpiece. Image courtesy of the Androscience. B) Normal sperm mitochondrial activity representative photograph with DAB dye accumulation in the sperm midpiece. Image courtesy of Androscience Lab. C) Example of coiled tail and amorphous spermatozoa in PCD spermatozoa. D) Representative photograph of defective DAB staining observed in PCD spermatozoa. Arrows demonstrate absence of mitochondrial activity by DAB stain and amorphous spermatozoa can be visualized.
Figure 2
Figure 2. A) Ultrastructural longitudinal section showing cross section of the sperm head and midpiece. Chromatin presents a granular appearance with hypodense lacunar defects. The mitochondrial sheath (left arrow) is seen as a conglomerate of ultra-structurally disorganized and overlapping mitochondria. Centriole cannot be properly found and correctly located. B) Transverse section of the sperm midpiece showing the initial part of the flagellum with its 9 outer dense fibers (arrow) and disorganized mitochondria sheath do not form a helically arranged single row (*). C) Transverse section of midpiece showing flagellum (arrow). The microtubular doublets of the axoneme are not clearly seen and the central pair is missing (dashed arrow). Disorganized mitochondria sheath does not form a helically arranged single row (*).
Figure 3
Figure 3. Ultrastructural images representing sperm flagella at the distal end of the principal piece. A and B) Four microtubular doublets of the axoneme are missing. The central pair and the peripheral doublets 1 to 5 are clearly visible (arrows). Note the absence of nexin links, outer and inner dynein arms in all doublets (*). C) Another sperm flagella. The central pair is missing (dashed arrow) and axonemal configuration is 9 + 0 (small arrows). D) Absence of doublets of axoneme and central pair (arrow). The fibrous sheath is thickened, disorganized and duplicated (*). E) Longitudinal section of the principal piece showing disorganized hyperplastic fibers of the fibrous sheath (arrows) and dense external longitudinal fibers (*).
Figure 4
Figure 4. Comparative ultrastructural morphology of normal spermatozoa, primary ciliary dyskinesia (PCD) and PCD with dysplasia of the fibrous sheath.
Illustration developed by Androscience.
See this image and copyright information in PMC

Comment in

References

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