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. 2021 Mar-Apr;35(2):827-831.
doi: 10.21873/invivo.12323.

Association of hsa-mir-328-3p Expression in Whole Blood With Optical Density of Retinal Pigment Epithelial Cells

Edita Kunceviciene  1 , Brigita Budiene  2 , Alina Smalinskiene  3 , Alvita Vilkeviciute  2 , Rasa Liutkeviciene  2   4
Affiliations

Association of hsa-mir-328-3p Expression in Whole Blood With Optical Density of Retinal Pigment Epithelial Cells

Edita Kunceviciene et al. In Vivo. 2021 Mar-Apr.

Abstract

Aim: To investigate the association of the pair box 6 gene (PAX6) and hsa-miR-328-3p with optical density of macular pigment.

Materials and methods: We evaluated 112 individuals (34 with moderate myopia, eight with high-degree myopia, and 70 healthy individuals). The optical density of macular pigment was measured using single-wavelength reflectometry. DNA and RNA were extracted from whole blood samples. Expression of hsa-miR-328-3p and genotyping of single-nucleotide polymorphism of PAX6 (rs662702) were performed using Applied Biosystems 7900HT real-time polymerase chain reaction system. Optical density of retinal pigment epithelial cells was evaluated using Fundus plus camera.

Results: In the group with myopia, with increasing ∆Ct hsa-miR-328-3p, the median optical density of the retinal pigment epithelium decreased statistically significantly (p<0.032). No statistically significant association was found between SNP rs662702 genotype variant of the PAX6 gene and the optical density of the retinal pigment epithelium.

Conclusion: The increased expression of hsa-miR-328-3p in the blood indicates a decrease in the optical density of the retinal pigment epithelium in those with myopia.

Keywords: PAX6; hsa-miR-328-3p; myopia; optical density; retinal pigment epithelial cell.

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Conflict of interest statement

Proprietary interests or conflicts: None of the Authors has any proprietary interests or conflicts of interest related to this submission. This submission has not been previously published anywhere, and it is not under consideration for any other publication.

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