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. 2020 Jul-Aug;23(4):384-389.
doi: 10.4103/JCD.JCD_376_20. Epub 2021 Jan 16.

Evaluation of the cytotoxic and genotoxic effects of different universal adhesive systems

Derya Sürmelioğlu  1 , Ceylan Hepokur  2 , Sevim Atılan Yavuz  3 , Uğur Aydın  1
Affiliations

Evaluation of the cytotoxic and genotoxic effects of different universal adhesive systems

Derya Sürmelioğlu et al. J Conserv Dent. 2020 Jul-Aug.

Abstract

Objectives: The objective of this study was to evaluate and compare the cytotoxicity and genotoxicity of different universal adhesive systems in the mouse fibroblast cell line L929.

Materials and methods: L929 (mouse fibroblast) cells were exposed to G-Premio Bond (GPB) (GC Europe, Belgium), Prime&Bond Universal (Dentsply Sirona, USA), Universal Bond Quick (Kuraray, USA), Single Bond (SB) Universal (3M ESPE, USA), and Tokuyama Universal Bond (TB) (Tokuyama, USA). Cell viability was assessed by the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide test, whereas oxidative DNA damage was assessed by determining the 8-hydroxydeoxyguanosine level using an enzyme-linked immunoassay kit. Statistical analysis was performed by one-way analysis of variance, followed by Bonferroni post hoc tests.

Results: Cytotoxic and genotoxic effects of TB and SB Universal groups were significantly higher than the other groups (P < 0.05). Among the adhesives tested, GPB (93.0 ± 1.3) had the least cytotoxicity, while TB (67.3 ± 3.0) had the most cytotoxic effect. In terms of genotoxicity, GPB (2.2 ± 0.3) had the least genotoxic effect, while Tokuyama Bond Universal (4.17 ± 0.4) had the most genotoxic effect.

Conclusions: Universal adhesive systems used in dentistry have cytotoxic and genotoxic effects in live cells. Universal adhesive systems should, therefore, be used with caution due to their cytotoxic and genotoxic effects in clinical applications.

Keywords: 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide; 8 OHdG level; adhesive systems; cytotoxicity; genotoxicity.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Cell viability (%) were represented for all groups and control
Figure 2
Figure 2
8-OHdG levels were represented for all groups and control
See this image and copyright information in PMC

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