Measurement of carbamazepine and its epoxide metabolite by high-performance liquid chromatography, and a comparison of assay techniques for the analysis of carbamazepine
- PMID: 336243
Measurement of carbamazepine and its epoxide metabolite by high-performance liquid chromatography, and a comparison of assay techniques for the analysis of carbamazepine
Abstract
We describe a modified high-performance liquid-chromatographic method for the simultaneous analysis of carbamazepine andits biologically active metabolite, carbamazepine-10, 11-epoxide. Concentrations of both these compounds in the plasma of 35 epileptic patients receiving chronic carbamazepine therapy are presented. Concentrations of carbamazepine in plasma were related to those of carbamazepine-10, 11-epoxide (r - 0.495, P less than 0.05). Total daily doses of carbamazepine were better correlated with plasma concentrations of carbamazepine-10, 11-epoxide (r = 0.714, P less than 0.001) than of carbamazepine (r = 0.269, P greater than 0.05). Close correlations were found between results of the three assay procedures we used to measure plasma carbamazepine concentrations: high-performance liquid chromatography, gas-liquid chromatography, and enzyme immunoassay. Correlation coefficients exceeded 0.97 and regression slopes were near unity, indicating that all three procedures were individually specific for the quantification of plasma carbamazepine.
Similar articles
-
Determination of carbamazepine and its epoxide metabolite in plasma by high-speed liquid chromatography.J Chromatogr. 1975 Jan 14;103(1):135-40. doi: 10.1016/s0021-9673(00)83807-8. J Chromatogr. 1975. PMID: 1117034
-
Competitive nephelometric immunoassay of carbamazepine and its epoxide metabolite in patient blood plasma.J Pharmacobiodyn. 1981 Jan;4(1):77-83. doi: 10.1248/bpb1978.4.77. J Pharmacobiodyn. 1981. PMID: 7277195
-
Plasma levels of carbamazepine and carbamazepine-10,11-epoxide during treatment of epilepsy.Eur J Clin Pharmacol. 1976 Mar 22;09(5-6):417-21. doi: 10.1007/BF00606558. Eur J Clin Pharmacol. 1976. PMID: 971704
-
Simultaneous liquid-chromatographic determination of carbamazepine and its epoxide metabolite in plasma.Clin Chem. 1982 Oct;28(10):2127-30. Clin Chem. 1982. PMID: 7127742
-
Routine monitoring of carbamazepine and carbamazepine-10,11-epoxide in plasma by high-performance liquid chromatography using 10-methoxycarbamazepine as internal standard.J Chromatogr. 1982 Aug 13;231(1):93-101. doi: 10.1016/s0378-4347(00)80512-5. J Chromatogr. 1982. PMID: 6749872
Cited by
-
Effect of cimetidine and ranitidine on carbamazepine and sodium valproate pharmacokinetics.Eur J Clin Pharmacol. 1984;27(3):341-3. doi: 10.1007/BF00542172. Eur J Clin Pharmacol. 1984. PMID: 6096154 Clinical Trial.
-
Effect of dosage frequency of carbamazepine on drug serum levels in epileptic patients.Eur J Clin Pharmacol. 1983;24(3):375-81. doi: 10.1007/BF00610058. Eur J Clin Pharmacol. 1983. PMID: 6861851
-
Effects of resins and activated charcoal on the absorption of digoxin, carbamazepine and frusemide.Br J Clin Pharmacol. 1988 Feb;25(2):229-33. doi: 10.1111/j.1365-2125.1988.tb03295.x. Br J Clin Pharmacol. 1988. PMID: 3358884 Free PMC article. Clinical Trial.
-
Saliva carbamazepine levels in children before and during multiple dosing.Br J Clin Pharmacol. 1982 May;13(5):647-51. doi: 10.1111/j.1365-2125.1982.tb01431.x. Br J Clin Pharmacol. 1982. PMID: 7082531 Free PMC article.
-
An immunodominant haptenic epitope of carbamazepine detected in serum from patients given long-term treatment with carbamazepine without allergic reaction.J Clin Immunol. 1992 Sep;12(5):335-40. doi: 10.1007/BF00920790. J Clin Immunol. 1992. PMID: 1430103