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. 2021 Feb;49(2):300060521989893.
doi: 10.1177/0300060521989893.

Performance of TDR-300B and VITEK®2 for the identification of Pseudomonas aeruginosa in comparison with VITEK®-MS

Lucky Hartati Moehario  1 , Enty Tjoa  1 , Hans Putranata  1 , Shikha Joon  2   3 , Daniel Edbert  1 , Thomas Robertus  4
Affiliations

Performance of TDR-300B and VITEK®2 for the identification of Pseudomonas aeruginosa in comparison with VITEK®-MS

Lucky Hartati Moehario et al. J Int Med Res. 2021 Feb.

Abstract

Objective: Automated systems are needed for the rapid and accurate diagnosis of Pseudomonas-associated nosocomial infections among critically ill patients admitted to the intensive care unit. We assessed the performance of TDR-300B and VITEK®2 for the identification of P. aeruginosa using VITEK®-MS as the gold standard.

Methods: This analytical study employed a cross-sectional approach. First, 44 clinical isolates of P. aeruginosa were collected and refreshed. Next, a single colony of oxidase-positive, gram-negative rods (30 samples) was inoculated into a TDR-300B NF-64 card and VITEK®2 GN cassette for each isolate. Finally, bacterial identification was performed using VITEK®-MS for comparative analysis.

Results: Compared with the results for VITEK®-MS, the congruence rates for TDR-300B and VITEK®2 were 80.76% (21/26) and 92.30% (24/26), respectively. Further, high sensitivity was observed for TDR-300B and VITEK®2 (95.45% and 100%, respectively). In addition, TDR-300B had a lower positive predictive value and accuracy than VITEK®2, albeit without significance.

Conclusions: Conclusively, there were no significant differences regarding the diagnostic efficiency of TDR-300B and VITEK®2 for P. aeruginosa.

Keywords: Automated systems; Pseudomonas aeruginosa; TDR-300B; VITEK®-MS; VITEK®2; nosocomial infections.

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Conflict of interest statement

Declaration of conflicting interest: The authors declare that there is no conflict of interest.

Figures

Figure 1.
Figure 1.
(a) TDR-300B and its (b) NF-64 card. (c) VITEK®2 and its (d) GN cassette NF, non-fermentative; GN, gram-negative.
See this image and copyright information in PMC

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