Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Feb;49(2):300060521989893.
doi: 10.1177/0300060521989893.

Performance of TDR-300B and VITEK®2 for the identification of Pseudomonas aeruginosa in comparison with VITEK®-MS

Lucky Hartati Moehario  1 , Enty Tjoa  1 , Hans Putranata  1 , Shikha Joon  2   3 , Daniel Edbert  1 , Thomas Robertus  4
Affiliations

Performance of TDR-300B and VITEK®2 for the identification of Pseudomonas aeruginosa in comparison with VITEK®-MS

Lucky Hartati Moehario et al. J Int Med Res. 2021 Feb.

Abstract

Objective: Automated systems are needed for the rapid and accurate diagnosis of Pseudomonas-associated nosocomial infections among critically ill patients admitted to the intensive care unit. We assessed the performance of TDR-300B and VITEK®2 for the identification of P. aeruginosa using VITEK®-MS as the gold standard.

Methods: This analytical study employed a cross-sectional approach. First, 44 clinical isolates of P. aeruginosa were collected and refreshed. Next, a single colony of oxidase-positive, gram-negative rods (30 samples) was inoculated into a TDR-300B NF-64 card and VITEK®2 GN cassette for each isolate. Finally, bacterial identification was performed using VITEK®-MS for comparative analysis.

Results: Compared with the results for VITEK®-MS, the congruence rates for TDR-300B and VITEK®2 were 80.76% (21/26) and 92.30% (24/26), respectively. Further, high sensitivity was observed for TDR-300B and VITEK®2 (95.45% and 100%, respectively). In addition, TDR-300B had a lower positive predictive value and accuracy than VITEK®2, albeit without significance.

Conclusions: Conclusively, there were no significant differences regarding the diagnostic efficiency of TDR-300B and VITEK®2 for P. aeruginosa.

Keywords: Automated systems; Pseudomonas aeruginosa; TDR-300B; VITEK®-MS; VITEK®2; nosocomial infections.

PubMed Disclaimer

Conflict of interest statement

Declaration of conflicting interest: The authors declare that there is no conflict of interest.

Figures

Figure 1.
Figure 1.
(a) TDR-300B and its (b) NF-64 card. (c) VITEK®2 and its (d) GN cassette NF, non-fermentative; GN, gram-negative.
See this image and copyright information in PMC

References

    1. Bentzmann S, Plésiat P. The Pseudomonas aeruginosa opportunistic pathogen and human infections. Environ Microbiol 2011; 13: 1655–1665. - PubMed
    1. Moradali MF, Ghods S, Rehm BH. Pseudomonas aeruginosa Lifestyle: A Paradigm for Adaptation, Survival, and Persistence. Front Cell Infect Microbiol 2017; 7: 39. - PMC - PubMed
    1. Revelas A. Healthcare-associated infections: A public health problem. Niger Med J 2012; 53: 59–64. - PMC - PubMed
    1. Estrada SR, Borgatta B, Rello J. Pseudomonas aeruginosa ventilator-associated pneumonia management. Infect Drug Resist 2016; 9: 7–18. - PMC - PubMed
    1. Kalanuria AA, Ziai W, Mirski M. Ventilator-associated pneumonia in the ICU. Crit Care 2014; 18: 208. - PMC - PubMed

MeSH terms

LinkOut - more resources