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. 2020 Jun;6(2):7-10.
doi: 10.18502/CMM.6.2.2694.

Does alternation of Candida albicans TUP1 gene expression affect the progress of symptomatic recurrent vulvovaginal candidiasis?

Mona Ghazanfari  1   2   2 , Azam Fattahi  3 , Mehraban Falahati  3 , Majid Bakhshizadeh  4 , Maryam Roudbary  1 , Faramarz Masjedian Jazi  5 , Mohsen Keykhosravi  6 , Ensieh Lotfali  7
Affiliations

Does alternation of Candida albicans TUP1 gene expression affect the progress of symptomatic recurrent vulvovaginal candidiasis?

Mona Ghazanfari et al. Curr Med Mycol. 2020 Jun.

Abstract

Background and purpose: Recurrent vulvovaginal candidiasis (RVVC) is one of the most common gynecological conditions in healthy and diabetic women, as well as antibiotic users. The present study was conducted to determine the relationship between TUP1 gene expression patterns and symptomatic recurrent C. albicans infections.

Materials and methods: This research was performed on C. albicans samples isolated from the vaginal specimens obtained from 31 individuals with RVVC in 2016. The reference strain C. albicans ATCC 10231, 10 C. albicans strains isolated from minimally symptomatic patients, and 10 isolates from asymptomatic patients were also used as control strains. The relative mRNA expression of the TUP1 gene was quantified using quantitative real-time polymerase chain reaction (QRT-PCR).

Results: The QRT-PCR results revealed that TUP1 mRNA expression was significantly decreased (0.001-0.930 fold) in the C. albicans isolates obtained from RVVC patients (P<0.001). However, no TUP1 expression was detectable in the isolates collected from asymptomatic patients. The results also indicated a significant correlation between TUP1 mRNA expression level and the severity of itching and discharge (P<0.001).

Conclusion: The present results were suggestive of the probable contribution of TUP1, as a part of the transcriptional repressor, to the severity of the symptoms related to C. albicans infections in the vagina. Regarding this, it is required to perform more in vivo studies using a larger sample size to characterize the regulatory or stimulatory function of TUP1 in the severity of RVVC symptoms. Furthermore, the study and identification of the genes involved in the severity of the symptomatic manifestations of C. albicans, especially in resistant strains, may lead to the recognition of an alternative antifungal target to enable the development of an effective agent.

Keywords: Expression; Filamentous growth; Vulvovaginal candidiasis; Candida albicans; TUP1 gene.

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Figures

Figure 1
Figure 1
Comparison of the expression of TUP1 at mRNA level between 31 samples and control group (P<0.001).
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References

    1. Adib SM, Bared EE, Fanous R, Kyriacos S. Practices of Lebanese gynecologists regarding treatment of recurrent vulvovaginal candidiasis. N Am J Med Sci. 2011; 3(9):406–10. - PMC - PubMed
    1. Lema VM. Recurrent vulvo-vaginal candidiasis: diagnostic and management challenges in a developing country context. Obstet Gynecol Int J. 2017; 7(5):260.
    1. Ghazanfari M, Falahati M, Fattahi A, Bazrafshan F, Nami S, Hosseinzadeh M, et al. Is MBL serum concentration a reliable predictor for recurrent vulvovaginal candidiasis? . Mycoses. 2017; 62(2):106–11. - PubMed
    1. Bernstein JA, Seidu L. Chronic vulvovaginal Candida hypersensitivity: an underrecognized and undertreated disorder by allergists. Allergy Rhinol. 2015; 6(1):44–9. - PMC - PubMed
    1. Rosentul DC, Delsing CE, Jaeger M, Plantinga TS, Oosting M, Costantini I, et al. Gene polymorphisms in pattern recognition receptors and susceptibility to idiopathic recurrent vulvovaginal candidiasis. Front Microbiol. 2014; 5:483. - PMC - PubMed

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