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Comparative Study
. 2021 May;13(5):1001-1007.
doi: 10.1002/dta.3021. Epub 2021 Mar 3.

Towards an untargeted mass spectrometric approach for improved screening in equine antidoping

Bethany Keen  1 , Adam Cawley  2 , Chris Fouracre  3 , James Pyke  4 , Shanlin Fu  1
Affiliations
Comparative Study

Towards an untargeted mass spectrometric approach for improved screening in equine antidoping

Bethany Keen et al. Drug Test Anal. 2021 May.

Abstract

The emergence of novel doping agents is a continuous issue for analysts who aim to maintain the integrity of horseracing together with the well-being and safety of the animals and riders involved. Untargeted mass spectrometric analysis presents a potential improvement for antidoping as it enables the detection of compounds being indirectly affected by an administered drug. In this study, liquid chromatography-high-resolution mass spectrometry was used to investigate a 12-horse administration study of the synthetic opioid, butorphanol. A mass spectrometric workflow capable of detecting metabolic differences for an extended period of time was successfully developed. This proof-of-concept study demonstrates the potential of untargeted workflows to provide a list of biomarkers of exposure and effect that are indicative of drug administration which may be implemented into routine testing for improved doping control.

Keywords: butorphanol; doping control; drug profiling; mass spectrometry.

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References

REFERENCES

    1. Lowry LK. Role of biomarkers of exposure in the assessment of health risks. Toxicol Lett. 1995;77(1):31-38.
    1. Nordberg GF. Biomarkers of exposure, effects and susceptibility in humans and their application in studies of interactions among metals in China. Toxicol Lett. 2010;192(1):45-49.
    1. Teale P, Barton C, Driver PM, Kay RG. Biomarkers: unrealized potential in sports doping analysis. Bioanalysis. 2009;1(6):1103-1118.
    1. Narduzzi L, Dervilly G, Audran M, Le Bizec B, Buisson C. A role for metabolomics in the antidoping toolbox? Drug Test Anal. 2020;12(6):677-690.
    1. Dettmer K, Aronov PA, Hammock BD. Mass spectrometry-based metabolomics. Mass Spectrom Rev. 2007;26(1):51-78.

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