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. 2021 Apr;18(4):1061-1063.
doi: 10.1038/s41423-021-00648-1. Epub 2021 Feb 25.

Emerging SARS-CoV-2 variants reduce neutralization sensitivity to convalescent sera and monoclonal antibodies

Jie Hu #  1 , Pai Peng #  1 , Kai Wang #  1 , Liang Fang  2 , Fei-Yang Luo  3 , Ai-Shun Jin  3 , Bei-Zhong Liu  4 , Ni Tang  5 , Ai-Long Huang  6
Affiliations

Emerging SARS-CoV-2 variants reduce neutralization sensitivity to convalescent sera and monoclonal antibodies

Jie Hu et al. Cell Mol Immunol. 2021 Apr.
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1. Neutralizing activities of convalescent sera and monoclonal antibodies against SARS-CoV-2 variants.
a Infectivity of WT and variant pseudoviruses assessed in 293T-ACE2 and A549-ACE2 cells. Cells were inoculated with equivalent doses of each pseudotyped virus. WT, wild-type Spike (GenBank: QHD43416) pseudotyped virus; Variant 1, N501Y.V1 mutant Spike pseudotyped virus (containing the H60/V70 and Y144 deletions and N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H mutations); Variant 2, N501Y.V2 mutant Spike pseudotyped virus (containing the K417N, E484K, N501Y, and D614G mutations). Neutralization of WT and variant pseudoviruses by convalescent sera. Pseudovirus-based neutralization assays were performed to detect neutralizing antibody (NAb) titers against SARS-CoV-2. The thresholds of detection were 1:40 of the ID50. Twenty sera (indicated by circles) were drawn 5 to 33 days post symptom onset (b); 20 sera (indicated by triangles) were drawn ~8 months post symptom onset (c). The half-maximal inhibitory concentrations (IC50) for tested monoclonal antibodies (mAbs) against pseudoviruses (d) and representative neutralization curves (e)
See this image and copyright information in PMC

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