Influence of seasonal temperature change on autotrophic nitrogen removal for mature landfill leachate treatment with high-ammonia by partial nitrification-Anammox process

Abstract

In this study, a denitrification (DN)-partial nitritation (PN)-anaerobic ammonia oxidation (Anammox) system for the efficient nitrogen removal of mature landfill leachate was built with a zone-partitioning self-reflux biological reactor as the core device, and the effects of changes in seasonal temperature on the nitrogen removal in non-temperature-control environment were explored. The results showed that as the seasonal temperature decreased from 34°C to 11.3°C, the total nitrogen removal rate of the DN-PN-Anammox system gradually decreased from the peak value of 1.42 kg/(m³•day) to 0.49 kg/(m³•day). At low temperatures (<20°C), when the nitrogen load (NLR) of the system is not appropriate, the fluctuation of high NH₄⁺-N concentration in the landfill leachate greatly influenced the stability of the nitrogen removal. At temperatures of 11°C-15°C, the NLR of the system is controlled below 0.5 kg/(m³•day), which can achieve stable nitrogen removal and the nitrogen removal efficiency can reach above 96%. The abundance of Candidatus Brocadia gradually increased with the decrease of temperature. Nitrosomonas, Candidatus Brocadia and Candidatus Kuenenia as the main functional microorganisms in the low temperature.

Keywords: Denitrification-partial nitrification-Anammox; Landfill leachate; Microbiology community; Nitrogen removal; Seasonal temperature.