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. 2021 Feb 27;14(1):75.
doi: 10.1186/s13104-021-05484-0.

Generating long-read sequences using Oxford Nanopore Technology from Diospyros celebica genomic DNA

Iskandar Zulkarnaen Siregar  1   2 , Fifi Gus Dwiyanti  3   4 , Rahadian Pratama  4   5 , Deden Derajat Matra  4   6 , Muhammad Majiidu  4
Affiliations

Generating long-read sequences using Oxford Nanopore Technology from Diospyros celebica genomic DNA

Iskandar Zulkarnaen Siregar et al. BMC Res Notes. .

Abstract

Objectives: Development of sequencing technology has opened up vast opportunities for tree genomic research in the tropics. One of the aforesaid technologies named ONT (Oxford Nanopore Technology) has attracted researchers in undertaking testings and experiments due to its affordability and accessibility. To the best of our knowledge, there has been no published reports on the use of ONT for genomic analysis of Indonesian tree species. This progress is promising for further improvement in order to acquire more genomic data for research purposes. Therefore, the present study was carried out to determine the effectiveness of ONT in generating long-read DNA sequences using DNA isolated from leaves and wood cores of Macassar ebony (Diospyros celebica Bakh.).

Data description: Long-read sequences data of leaves and wood cores of Macassar ebony were generated by using the MinION device and MinKnow v3.6.5 (ONT). The obtained data, as the first long-read sequence dataset for Macassar ebony, is of great importance to conserve the genetic diversity, understanding the molecular mechanism, and sustainable use of plant genetic resources for downstream applications.

Keywords: Diospyros celebica; Genome; Long-read sequences; ONT; Scaffolds.

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Conflict of interest statement

The authors declare that they have no competing interests.

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