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. 2021 Jan;24(1):85-91.
doi: 10.22038/ijbms.2020.50621..

Neurochemical effects of sleep deprivation in the hippocampus of the pilocarpine-induced rat model of epilepsy

Heba S Aboul Ezz  1 , Aboul Ezz Noor  1 , Iman M Mourad  1 , Heba Fahmy  2 , Yasser A Khadrawy  3
Affiliations

Neurochemical effects of sleep deprivation in the hippocampus of the pilocarpine-induced rat model of epilepsy

Heba S Aboul Ezz et al. Iran J Basic Med Sci. 2021 Jan.

Abstract

Objectives: The present study aims to investigate the pathological mechanisms mediating the effect of paradoxical sleep deprivation (PSD) for 48 hr on the spontaneous recurrent seizures (SRS) stage of the pilocarpine rat model of temporal lobe epilepsy.

Materials and methods: This was carried out through assessment of amino acid neurotransmitter levels, the main oxidative stress parameters, and the levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in the hippocampus. The experimental animals were divided into 4 groups: control, epileptic, PSD, and epileptic+PSD groups.

Results: Data indicated that PSD in epileptic rats induced a significant decrease in GSH levels. TNF-α increased significantly in the PSD group and decreased significantly in both epileptic rats and epileptic rats deprived of paradoxical sleep. PSD induced a significant increase in glutamine, glutamate, and aspartate and a significant decrease in GABA. In epileptic rats and epileptic rats deprived of PS, a significant increase in aspartate and a significant decrease in GABA and taurine were recorded.

Conclusion: The present data suggest that exposure to PSD for 48 hr did not worsen the alterations produced in the present epileptic model. However, epileptic, PSD, epileptic + PSD groups showed a state of hyperexcitability and oxidative stress. PSD may increase the susceptibility of animals to the development of epilepsy.

Keywords: Amino acids; Cytokines; Epilepsy; Hippocampus; Oxidative stress; Sleep deprivation.

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Figures

Figure 1
Figure 1
Effect of paradoxical sleep deprivation for 48 hr on the levels of lipid peroxidation (MDA), reduced glutathione (GSH), nitric oxide (NO), and acetylcholinesterase activity (AchE) in the hippocampus of control and epileptic rats
Figure 2
Figure 2
Effect of paradoxical sleep deprivation for 48 hr on the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (Int.6), and interleukin 1β (Int.1β) in the hippocampus of control and epileptic rats
See this image and copyright information in PMC

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