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. 2021 Feb 6:35:106852.
doi: 10.1016/j.dib.2021.106852. eCollection 2021 Apr.

Raw pacific biosciences and illumina sequencing reads and assembled genome data for the cattle ticks Rhipicephalus microplus and Rhipicephalus annulatus

Felix D Guerrero  1 , Noushin Ghaffari  2 , Kylie G Bendele  1 , Richard P Metz  3 , C Michael Dickens  4 , Philip D Blood  5 , Jason Tidwell  6 , Robert J Miller  6 , Adalberto A Pérez de León  1 , Pete D Teel  7 , Charles D Johnson  3
Affiliations

Raw pacific biosciences and illumina sequencing reads and assembled genome data for the cattle ticks Rhipicephalus microplus and Rhipicephalus annulatus

Felix D Guerrero et al. Data Brief. .

Abstract

Ticks from the genus Rhipicephalus have enormous global economic impact as ectoparasites of cattle. Rhipicephalus microplus and Rhipicephalus annulatus are known to harbor infectious pathogens such as Babesia bovis, Babesia bigemina, and Anaplasma marginale. Having reference quality genomes of these ticks would advance research to identify druggable targets for chemical entities with acaricidal activity and refine anti-tick vaccine approaches. We sequenced and assembled the genomes of R. microplus and R. annulatus, using Pacific Biosciences and HiSeq 4000 technologies on very high molecular weight genomic DNA. We used 22 and 29 SMRT cells on the Pacific Biosciences Sequel for R. microplus and R. annulatus, respectively, and 3 lanes of the Illumina HiSeq 4000 platform for each tick. The PacBio sequence yields for R. microplus and R. annulatus were 21.0 and 27.9 million subreads, respectively, which were assembled with Canu v. 1.7. The final Canu assemblies consisted of 92,167 and 57,796 contigs with an average contig length of 39,249 and 69,055 bp for R. microplus and R. annulatus, respectively. Annotated genome quality was assessed by BUSCO analysis to provide quantitative measures for each assembled genome. Over 82% and 92% of the 1066 member BUSCO gene set was found in the assembled genomes of R. microplus and R. annulatus, respectively. For R. microplus, only 189 of the 1066 BUSCO genes were missing and only 140 were present in a fragmented condition. For R. annulatus, only 75 of the BUSCO genes were missing and only 109 were present in a fragmented condition. The raw sequencing reads and the assembled contigs/scaffolds are archived at the National Center for Biotechnology Information.

Keywords: Canu assembler; Cattle tick; Large genome assembly; PacBio genome sequencing; Rhipicephalus annulatus; Rhipicephalus microplus.

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Conflict of interest statement

This work was funded in parts by the USDA-ARS CRIS Project No. 3094-32000-036-00D, a USDA-ARS Cooperative Agreement No. 58-3094-6-017 with the Department of Entomology, Texas A&M AgriLife Research, College Station, TX, USA, and by Texas A&M AgriLife Research through an Insect Vector Diseases Competitive Grant and High Consequence Genomics Research Project on Vector-borne Diseases to the Department of Entomology. This work used the Extreme Science and Engineering Discovery Environment (XSEDE), which is supported by National Science Foundation grant number ACI-1548562. Specifically, it used the Bridges system, which is supported by NSF award number ACI-1445606, at the Pittsburgh Supercomputing Center (PSC).

Figures

Fig 1
Fig. 1
Flow diagram of the genome sequencing, assembly, quality assessment, and annotation process.
See this image and copyright information in PMC

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