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. 2021 Apr;106(4):e21776.
doi: 10.1002/arch.21776. Epub 2021 Feb 28.

Unique Wolbachia strain wMelPlus increases heat stress resistance in Drosophila melanogaster

Elena V Burdina  1 , Roman A Bykov  1 , Petr N Menshanov  1   2   3 , Yury Yu Ilinsky  1 , Nataly Е Gruntenko  1
Affiliations

Unique Wolbachia strain wMelPlus increases heat stress resistance in Drosophila melanogaster

Elena V Burdina et al. Arch Insect Biochem Physiol. 2021 Apr.

Abstract

Maternally inherited endosymbiotic bacterium Wolbachia infects Drosophila melanogaster populations worldwide. Its genetic diversity includes several closely related genotypes, which can be attributed to two main genotype groups: wMel and wMelCS. Here, we studied eight D. melanogaster lines carrying the nuclear background of wild type interbred Bi90 line and cytoplasmic backgrounds with or without Wolbachia of different origin, each of which belongs to wMelCS genotype group. We analyzed the effect these seven Wolbachia strains had on the heat stress resistance and dopamine metabolism in D. melanogaster females. Survival under heat stress (38°C, 3 h 30 min) was increased only in the line infected with bacteria of the wMelPlus strain. At the same time, the activity of alkaline phosphatase (an enzyme regulating the pool of dopamine precursor tyrosine) was increased under normal conditions in females infected with all strains under study and retained the response to heat stress typical for the uninfected line. Thus, we found the unique Wolbachia strain that provides an increase of the host stress resistance, and demonstrated that the mechanism of this resistance is not dopamine-mediated.

Keywords: Drosophila; Wolbachia; alkaline phosphatase; heat stress; stress resistance.

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References

REFERENCES

    1. Adonyeva, N. V., Burdina, E. V., Bykov, R. A., Gruntenko, N. E., & Rauschenbach, I. Y. (2019). Genotype of Wolbachia pipientis endosymbiont affects octopamine metabolism in Drosophila melanogaster females. Russian Journal of Genetics, 55, 653-655.
    1. Bogomolova, E. V., Rauschenbach, I. Y., Adonyeva, N. V., Alekseev, A. A., Faddeeva, N. V., & Gruntenko, N. E. (2010). Dopamine down-regulates activity of alkaline phosphatase in Drosophila: the role of D2-like receptors. Journal of Insect Physiology, 56, 1155-1159.
    1. Bykov, R. А., Yudina, M. A., Gruntenko, N. E., Zakharov, I. K., Voloshina, M. A., Melashchenko, E. S., Danilova, M. V., Mazunin, I. O., & Ilinsky, Y. Y. (2019). Prevalence and genetic diversity of Wolbachia endosymbiont and mtDNA in Palearctic populations of Drosophila melanogaster. BMC Evolutionary Biology, 19(Suppl 1), 48.
    1. Gruntenko, N. E., Chentsova, N. A., Bogomolova, E. V., Karpova, E. K., Glazko, G. V., Faddeeva, N. V., Monastirioti, M., & Rauschenbach, I. Y. (2004). The effect of mutations altering biogenic amine metabolism in Drosophila on viability and the response to environmental stresses. Archives of Insect Biochemistry and Physiology, 55(2), 55-67.
    1. Gruntenko, N. E., Karpova, E. K., Adonyeva, N. V., Andreenkova, O. V., Burdina, E. V., Ilinsky, Y. Y., Bykov, R. A., Menshanov, P. N., & Rauschenbach, I. Y. (2019). Drosophila female fertility and juvenile hormone metabolism depends on the type of Wolbachia infection. JEB, 222, jeb195347.

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