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. 2021 Mar;64(3):391-394.
doi: 10.1165/rcmb.2020-0137LE.

Nonclassical Monocytes Promote Edema in Lung Allografts from Traumatic Brain Injury Donors

Wenbin Yang  1 , Stephen Chiu  1 , Melissa Querrey  1 , Xianpeng Liu  1 , Qiang Wu  1 , Emily Cerier  1 , Mecca B A R Islam  1 , Steven J Schwulst  1 , G R Scott Budinger  1 , Thalachallour Mohanakumar  2 , Emilia Lecuona  1 , Ankit Bharat  1
Affiliations

Nonclassical Monocytes Promote Edema in Lung Allografts from Traumatic Brain Injury Donors

Wenbin Yang et al. Am J Respir Cell Mol Biol. 2021 Mar.
No abstract available

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Figures

Figure 1.
Figure 1.
Nonclassical monocytes (NCM) promote lung edema from traumatic brain injury (TBI). Mouse brains were harvested for determination of (A) morphology and (B) neurological severity score, from sham and TBI mice (n = 4–5). (C) Lungs were harvested for determination of pulmonary edema by wet-to-dry ratio measurement following pharmacological and genetic depletion of the different subset of monocytes (n = 3–7). (D) Number of NCM and NK cells following TBI in mice (n = 3). (E) The ratio of NCM/AM in the lungs isolated from human non-TBI and TBI donors. Data expressed as cell count per alveolar macrophage (AM) to standardize across patients (n = 10). (F) NCM isolated from mouse lung secrete TNF-α in response to HMGB1 (n = 4–5). All graphs show means ± SD. Graph on (C) was analyzed by one-way ANOVA followed by Tukey’s post hoc test. All other graphs were analyzed by two-tailed unpaired t test. *P < 0.05, **P < 0.01, and ****P < 0.0001. B/L = bilateral; HMGB1 = high-mobility group box-1 protein; NK = natural killer; n.s. = nonsignificant.
Figure 2.
Figure 2.
TBI is associated with worse allograft function following both murine and human lung transplantation. Allograft function measured by PaO2/FiO2 ratio at 24 hours after transplant of human (n = 10) (A) and murine (B) lungs (n = 5–12) is shown. (A) Following human lung transplantation, the function of the allograft was analyzed by obtaining the blood directly from the pulmonary vein of the allograft. (B) For murine lung transplantation, the blood was obtained from the aorta after the hilum of the native right lung was clamped for 5 minutes and the host was on 100% FiO2. Graphs show means ± SD (A) analyzed by two-tailed unpaired t test and (B) by one-way ANOVA followed by Tukey’s post hoc test. *P < 0.05. PaO2/FiO2 = arterial oxygen pressure/fraction of inspired oxygen.
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References

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