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. 2021 Jul;28(26):34300-34308.
doi: 10.1007/s11356-021-13051-0. Epub 2021 Mar 1.

Enhancement of exopolysaccharides production and reactive oxygen species level of Nostoc flagelliforme in response to dehydration

Shijie Wu  1   2   3 , Kaiqiang Yu  1   3 , Long Li  4 , Lingxia Wang  3 , Wenyu Liang  5
Affiliations

Enhancement of exopolysaccharides production and reactive oxygen species level of Nostoc flagelliforme in response to dehydration

Shijie Wu et al. Environ Sci Pollut Res Int. 2021 Jul.

Abstract

Nostoc flagelliforme is a remarkable drought-resistant terrestrial cyanobacterium whose exopolysaccharides (EPS) have been found to exert important physiological and ecological functions, and the EPS are known to improve soil physicochemical properties. In this study, we used physiological and molecular methods to investigate the influences of three moisture loss levels on EPS production and the antioxidant system in N. flagelliforme. The aim was to reveal the EPS production mechanism involved in the gene differential expression and antioxidant system of N. flagelliforme in response to drought. Our results showed that EPS contents increased by 13% and 22% after 6-h and 48-h dehydration (6HAD and 48HAD) compared with 4-h rehydration (4HAR), respectively. The same trends were also detected for most EPS synthesis genes, especially glycosyltransferases. Furthermore, the intracellular reactive oxygen species (ROS) levels in N. flagelliforme were generally higher at 6HAD and 48HAD than at 4HAR. Superoxide dismutase (SOD) and peroxidase (POD) activities were restricted in N. flagelliforme under 6HAD and 48HAD compared with 4HAR, but the opposite result was found in catalase (CAT) activity. These results provide a new foundation for understanding the mechanism of EPS accumulation in N. flagelliforme in response to drought.

Keywords: Antioxidant enzymes; Drought tolerance; Exopolysaccharides; Gene differential expression; Nostoc flagelliforme; ROS.

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References

    1. Adessi A, Cruz de Carvalho R, De Phillippis R, Branquinho C, Marques D, Silva J (2018) Microbial extracellular polymeric substances improve water retention in dryland biological soil crusts. Soil Biol Biochem 116:67–69 - DOI
    1. Ahmada B, Azeema F, Alib MA, Nawazd MA, Nadeema H, Abbasb A, Batoole R, Atiff RM, Ijaza U, Nieves-Cordonesh M, Chung G (2020) Genome-wide identification and expression analysis of two component system genes in Cicer arietinum. Genomics 112:1371–1383 - DOI
    1. Anders S (2010) HTSeq: analysing high-throughput sequencing data with Python.
    1. Anders S, Huber W (2012) Differential expression of RNA-Seq data at the gene level-the DESeq package. European Molecular Biology Laboratory (EMBL), Heidelberg. pp 24.
    1. Belnap J, Phillips SL, Flint SD, Money J, Caldwell MM (2008) Global change and biological soil crusts: effects of ultraviolet augmentation under altered precipitation regimes and nitrogen additions. Glob Chang Biol 14:670–686 - DOI

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