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Comment
. 2021 Mar;18(3):246-248.
doi: 10.1038/s41592-021-01083-w. Epub 2021 Mar 1.

Reply to: Limitations of the iterative electron density reconstruction algorithm from solution scattering data

Thomas D Grant  1
Affiliations
Comment

Reply to: Limitations of the iterative electron density reconstruction algorithm from solution scattering data

Thomas D Grant. Nat Methods. 2021 Mar.
No abstract available

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Figures

Fig. 1 ∣
Fig. 1 ∣. Calculated or reconstructed low-resolution density of DDM micelles and protein–nucleic acid complexes.
a, Reconstructions from SAXS data of DDM micelles with large interior negative contrast. The average of 20 DENSS reconstructions is shown as a 3D transparent volume (left) and a cross-section (right). Density is colored in green (positive density) and red (negative density). b, Calculated density from atomic coordinates at low resolution using EMAN2 (ref. ) for the protein–nucleic acid complexes shown by Konarev and Svergun. Maps were calculated at the resolution shown in the figure as estimated by DENSS reconstructions. Maps are colored from low density (blue) to high density (red). c, Calculated and reconstructed electron density maps of the modified protein–DNA complex for PDB ID 5Y0D with the same coloring as in b.
See this image and copyright information in PMC

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References

    1. Konarev PV & Svergun DI Limitations of the iterative electron density reconstruction algorithm from solution scattering data. Nat. Methods 10.1038/s41592-021-01082-x (2021). - DOI - PMC - PubMed
    1. Grant TD Ab initio electron density determination directly from solution scattering data. Nat. Methods 15, 191–193 (2018). - PubMed
    1. Garavito RM & Ferguson-Miller S Detergents as tools in membrane biochemistry. J. Biol. Chem 276, 32403–32406 (2001). - PubMed
    1. Hyland LL, Taraban MB & Yu YB Using small-angle scattering techniques to understand mechanical properties of biopolymer-based biomaterials. Soft Matter 10.1039/C3SM51209F (2018). - DOI - PMC - PubMed
    1. Manet S et al. Structure of micelles of a nonionic block copolymer determined by SANS and SAXS. J. Phys. Chem. B 115, 11318–11329 (2011). - PubMed

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