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Comment
. 2021 Mar;18(3):244-245.
doi: 10.1038/s41592-021-01082-x. Epub 2021 Mar 1.

Limitations of the iterative electron density reconstruction algorithm from solution scattering data

Petr V Konarev  1 , Dmitri I Svergun  2
Affiliations
Comment

Limitations of the iterative electron density reconstruction algorithm from solution scattering data

Petr V Konarev et al. Nat Methods. 2021 Mar.
No abstract available

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Conflict of interest statement

Competing Interests

The authors declare no competing interests.

Figures

Figure 1
Figure 1
DENSS reconstructions from theoretical scattering data computed up to a nominal resolution of 12 Å. Averaging was done over 100 reconstructions by the ‘superdenss’ script. Two-dimensional slices are coloured from red (higher density, relative value of 20) to blue (lower density, value 5). The threshold to display was selected to ensure the best shape overlap between the initial models and DENSS maps; bar length, 50 Å. (A) Protein-DNA complexes (PDB codes 1B3T, 4E54, 5Y0D and 6FTX), and two protein models, with PDB codes 6PWC (A and R chains) and 6KYK (A and D chains), with artificially doubled contrast of one domain (R and D chains, respectively). The crystal structures are displayed as cartoons (protein, blue; DNA and doubled domains, red). (B) Ellipsoids of revolution: top row, model particles (left, uniform ellipsoid; middle and right, non-uniform ellipsoids with densities 1 (blue) and 2 (red)). Bottom row, restored densities.
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References

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