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. 2021:2278:141-148.
doi: 10.1007/978-1-0716-1274-3_12.

Identification of Bifidobacteria by the Phosphoketolase Assay

Monica Modesto  1 , Alice Checcucci  1 , Paola Mattarelli  2
Affiliations

Identification of Bifidobacteria by the Phosphoketolase Assay

Monica Modesto et al. Methods Mol Biol. 2021.

Abstract

Bifidobacteria are commensal bacteria, which naturally colonize the gastrointestinal tract of a large number of animals, including humans, contributing to their health and well-being. An important taxonomic marker for the identification of members of the bifidobacterial group is the presence of the fructose-6-phosphate phosphoketolase (F6PPK) activity. The F6PPK enzyme is involved in the bifidus shunt based on the ability of F6PPK to split fructose-6-phosphate into erythrose-4-phosphate and acetyl phosphate. Here, we describe the two main methods utilized to detect the presence of F6PPK activity, that is, the enzymatic assay and the presence of the D-xylulose-5-phosphate/fructose-6-phosphate phosphoketolase bifidobacterial gene.

Keywords: Bacterial taxonomy; Bifidobacterium; Bifidus shunt; Fructose 6 phosphoketolase; Polymerase chain reaction.

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