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. 2021 Jun;72(6):741-749.
doi: 10.1111/lam.13468. Epub 2021 Mar 6.

High concentration of sodium chloride could induce the viable and culturable states of Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidis

Hana Song  1 , Sun-Young Lee  1
Affiliations

High concentration of sodium chloride could induce the viable and culturable states of Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidis

Hana Song et al. Lett Appl Microbiol. 2021 Jun.

Abstract

In the present study, Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidis were transferred into Luria-Bertani medium without NaCl (LBWS) and adjusted to various pHs (4, 5, 6 and 7) with lactic acid containing 0·75, 5, 10 and 30% NaCl, and stored at 25°C until the bacterial populations reached below detectable levels on tryptic soy agar (TSA). Although E. coli O157:H7 and S. Enteritidis did not grow on TSA when incubated in LBWS with 30% NaCl for 35 and 7 days, more than 60 and 70% of the bacterial cells were shown to be viable via fluorescent staining with SYTO9 and propidium iodide (PI), respectively, suggesting that a number of cells could be induced into the viable but nonculturable (VBNC) state. These bacteria that were induced into a VBNC state were transferred to a newly prepared tryptic soy broth (TSB) and then incubated at 37°C for several days. After more than 7 days, E. coli O157:H7 and S. Enteritidis regained their culturability. We, therefore, suggest that E. coli O157:H7 and S. Enteritidis entered the VBNC state under the adverse condition of higher salt concentrations and were revived when these conditions were reversed.

Keywords: Escherichia coli O157; H7; NaCl; Salmonella enterica serovar Enteritidis; VBNC; pH; survival.

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References

    1. Bae, Y.M. and Lee, S.Y. (2015) Combined effects of organic acids and salt depending on type of acids and pathogens in laboratory media and acidified pickle. J Appl Microbiol 119, 455-464.
    1. Beuchat, L.R. and Mann, D.A. (2015) Survival of Salmonella in cookie and cracker sandwiches containing inoculated, low-water activity fillings. J Food Prot 78, 1828-1834.
    1. Chen, S., Li, X., Wang, Y., Zeng, J., Ye, C., Li, X., Guo, L., Zhang, S. et al. (2018) Induction of Escherichia coli into a VBNC state through chlorination/chloramination and differences in characteristics of the bacterium between states. Water Res 142, 279-288.
    1. Chen, W., Golden, D.A., Critzer, F.J. and Davidson, P.M. (2015) Antimicrobial activity of cinnamaldehyde, cavacrol, and lauric arginate against Salmonella Tennessee in a glycerol-sucrose model and peanut paste at different fat concentrations. J Food Prot 78, 1488-1495.
    1. Cho, T.J., Kim, N.H., Song, J.H. and Rhee, M.S. (2016) Survival of foodborne pathogens (Escherichia coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus, Listeria monocytogenes, and Vibrio parahaemolyticus) in raw ready-to-eat crab marinated in soy sauce. Int J Food Microbiol 238, 50-55.

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