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. 2019 Oct 5;9(19):e3380.
doi: 10.21769/BioProtoc.3380.

A Novel Protocol to Generate Decellularized Bovine Spinal Cord Extracellular Matrix-Based Scaffolds (3D-dCBS)

Yavuz E Arslan  1 , Burcu Efe  1 , Tugba Sezgin Arslan  1
Affiliations

A Novel Protocol to Generate Decellularized Bovine Spinal Cord Extracellular Matrix-Based Scaffolds (3D-dCBS)

Yavuz E Arslan et al. Bio Protoc. .

Abstract

Extracellular matrix (ECM)-based tissue engineering scaffolds have an essential role in promoting tissue regeneration. Nerve tissue engineering aims at facilitating the repair of permanent damage to the peripheral and central nervous systems, which are difficult to heal. For this purpose, a variety of biomaterials are being developed consisting of numerous synthetic and/or natural polymers to provide axonal reinnervation and to direct the growth of axons. Here, we present a novel protocol that enables to fabricate a 3-dimensional (3D) decellularized scaffold derived from the bovine spinal cord (BSC) ECM (3D-dCBS) for neural tissue engineering applications. In this protocol, a viscous ECM-derived gel from BSC is prepared, molded, and chemically crosslinked with EDC/NHS (3D-CBS) before decellularization process. Decellularization of 3D-CBS is performed with 1% SDS to attain 3D-dCBS. As compared with other available methods, our protocol is a novel decellularization method that preserves a more significant part of the ECM. We believe that the mentioned protocol has the potential to produce a bioengineered scaffold from spinal cord tissue with desired geometry for regenerative medicine applications related to neural tissue engineering.

Keywords: Bovine spinal cord; Decellularized nerve scaffold; Neural graft; Regenerative medicine; Tissue reconstruction.

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Conflict of interest statement

Competing interestsThe authors have declared that no competing interests exist.

Figures

Figure 1.
Figure 1.. 3D-dCBS fabrication process
See this image and copyright information in PMC

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