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. 2021 Apr 20;59(5):e00329-21.
doi: 10.1128/JCM.00329-21. Print 2021 Apr 20.

New Pneumococcal Serotype 15D

Fabiana Pimenta  1 , Benild Moiane  2 , Robert E Gertz Jr  1 , Sopio Chochua  1 , Paula M Snippes Vagnone  3 , Ruth Lynfield  3 , Betuel Sigaúque  2 , Maria da Gloria Carvalho  1 , Bernard Beall  4
Affiliations

New Pneumococcal Serotype 15D

Fabiana Pimenta et al. J Clin Microbiol. .
No abstract available

Keywords: Streptococcus pneumoniae; capsular polysaccharide; vaccines.

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Figures

FIG 1
FIG 1
Alignment of cps operons from the four known serogroup 15 serotypes and from the two isolates expressing the new serotype 15D. The three genes indicated in red provide basis by which to resolve the five different serotypes shown. The gray open reading frames encode enzymes that have functions unrelated to serogroup 15 polysaccharide structure. The short black open reading frames are transposase gene remnants. The two serotype 15D strains were subjected to whole-genome sequencing as previously described (1, 2). Sequences encompassing the dexB 3′ end and the aliA 5′ end were extracted from these two strains and were aligned with published GenBank sequences for cps15F (CR931666), cps15A (CR931663), cps15B (CR931664), and 15C (CR931665) (see references and 6). The extracted sequences and published serogroup 15 cps operons were subjected to Prokka to identify and annotate open reading frames (9). The resultant annotated sequences were analyzed by BLAST and the homolog cps locus regions were aligned into figures using EasyFig version 2.2.3 software (10). Strains 84245 and MZ877 were assigned BioSample accession numbers SAMN14150919 and SAMN17684515, respectively, under project number PRJNA697931.
See this image and copyright information in PMC

References

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    1. van Selm S, van Cann LM, Kolkman MA, van der Zeijst BA, van Putten JP. 2003. Genetic basis for the structural difference between Streptococcus pneumoniae serotype 15B and 15C capsular polysaccharides. Infect Immun 71:6192–6198. 10.1128/iai.71.11.6192-6198.2003. - DOI - PMC - PubMed

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