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. 1977 Dec;132(3):1027-30.
doi: 10.1128/jb.132.3.1027-1030.1977.

Reconstitution and purification of flagellar filaments from Caulobacter crescentus

M Sheffery, A Newton

Reconstitution and purification of flagellar filaments from Caulobacter crescentus

M Sheffery et al. J Bacteriol. 1977 Dec.

Abstract

Filaments from isolated flagella of Caulobacter crescentus have been purified by successive dissociation and reconstitution. After the second and third reconstitutions from subunits in 0.8 M sodium citrate, filament preparations contained only two proteins, flagellin A (26,000 daltons) and flagellin B (28,000 daltons). There was some enrichment for flagellin A during reconstitution by this procedure, since isolated flagella contained flagellin A and flagellin B in a ratio of approximately 3.8:1 and filaments after the third reconstitution contained the two proteins in a ratio of 5.0:1.

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References

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