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. 1977 Dec;132(3):1036-7.
doi: 10.1128/jb.132.3.1036-1037.1977.

Selection of yeast auxotrophs by thymidylate starvation

B J Barclay, J G Little

Selection of yeast auxotrophs by thymidylate starvation

B J Barclay et al. J Bacteriol. 1977 Dec.

Abstract

A rapid procedure for the recovery of Saccharomyces cerevisiae auxotrophs was developed by exploiting the protection of these mutants from thymineless death when a required metabolite was withheld. The method can be used for thymidine 5'-monophosphate-requiring auxotrophs or wild-type strains blocked in de novo synthesis of thymidylate by folate antagonists.

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References

    1. Mol Gen Genet. 1975 Dec 30;143(1):5-11 - PubMed

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