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. 1977 Nov;6(5):439-44.
doi: 10.1128/jcm.6.5.439-444.1977.

Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin

R H Yolken, H B Greenberg, M H Merson, R B Sack, A Z Kapikian

Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin

R H Yolken et al. J Clin Microbiol. 1977 Nov.

Abstract

The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity between Vibrio cholerae toxin and heat-labile E. coli enterotoxin, is similar in design to a radioimmunoassay but utilizes enzyme-labeled rather than radioactive isotope-labeled reagents. The ELISA system is as sensitive as both radioimmunoassay and the y-1 adrenal cell assay for the detection of heat-labile E. coli enterotoxin but requires neither radioactive reagents nor tissue culture techniques. The ELISA is easy to perform and is adaptable for use in small laboratories.

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